Detailed information NTG0990080

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHW32_RS22175
• Genome accession: NZ_CP152911
• Coordinates: 4517936..4518736 (+)
• Length: 266 a.a.
• NCBI ID: WP_023292017.1
• Uniprot ID: A0A483KIE1
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain NK_H13_003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4512936..4523736

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHW32_RS22140 (AAHW32_22115)	gspH	4513089..4513604 (+)	516	WP_060591174.1	type II secretion system minor pseudopilin GspH	-
AAHW32_RS22145 (AAHW32_22120)	gspI	4513601..4513966 (+)	366	WP_032452553.1	type II secretion system minor pseudopilin GspI	-
AAHW32_RS22150 (AAHW32_22125)	gspJ	4513963..4514559 (+)	597	WP_023292022.1	type II secretion system minor pseudopilin GspJ	-
AAHW32_RS22155 (AAHW32_22130)	gspK	4514556..4515536 (+)	981	WP_077259115.1	type II secretion system minor pseudopilin GspK	-
AAHW32_RS22160 (AAHW32_22135)	gspL	4515529..4516725 (+)	1197	WP_048296714.1	type II secretion system protein GspL	-
AAHW32_RS22165 (AAHW32_22140)	-	4516718..4517203 (+)	486	WP_049014314.1	type II secretion system protein M	-
AAHW32_RS22170 (AAHW32_22145)	-	4517193..4517921 (+)	729	WP_048296713.1	type II secretion system protein N	-
AAHW32_RS22175 (AAHW32_22150)	pilD	4517936..4518736 (+)	801	WP_023292017.1	A24 family peptidase	Machinery gene
AAHW32_RS22180 (AAHW32_22155)	hrpB	4518784..4521213 (-)	2430	WP_374416475.1	ATP-dependent helicase HrpB	-
AAHW32_RS22185 (AAHW32_22160)	thpR	4521286..4521822 (+)	537	WP_060591166.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHW32_RS22190 (AAHW32_22165)	sfsA	4521822..4522538 (+)	717	WP_023292014.1	DNA/RNA nuclease SfsA	-
AAHW32_RS22195 (AAHW32_22170)	dksA	4522701..4523156 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29274.82 Da
• Isoelectric Point: 8.8894

>NTDB_id=990080 AAHW32_RS22175 WP_023292017.1 4517936..4518736(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain NK_H13_003]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDEPITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELATGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLLASASGLAWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990080 AAHW32_RS22175 WP_023292017.1 4517936..4518736(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain NK_H13_003]
ATGACGACATTAGCCGCATTATCGCTGCATTTTCCCCTTGTCTGGTATGGTTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGACCCAAACCGCGGACGACGAGCCGATAACCCTCAGCA
CGCCGGGCTCATCCTGCCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCCCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGTTGGC
AGGCTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCAGCGGGCTGGCGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A483KIE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425