Detailed information NTG0989733

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHX35_RS22110
• Genome accession: NZ_CP152637
• Coordinates: 4580124..4580924 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain NK_H29_003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4575124..4585924

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHX35_RS22075 (AAHX35_22050)	gspH	4575277..4575792 (+)	516	WP_374184370.1	type II secretion system minor pseudopilin GspH	-
AAHX35_RS22080 (AAHX35_22055)	gspI	4575789..4576154 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHX35_RS22085 (AAHX35_22060)	gspJ	4576151..4576747 (+)	597	WP_374184371.1	type II secretion system minor pseudopilin GspJ	-
AAHX35_RS22090 (AAHX35_22065)	gspK	4576744..4577724 (+)	981	WP_374184372.1	type II secretion system minor pseudopilin GspK	-
AAHX35_RS22095 (AAHX35_22070)	gspL	4577717..4578913 (+)	1197	WP_374184373.1	type II secretion system protein GspL	-
AAHX35_RS22100 (AAHX35_22075)	-	4578906..4579391 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHX35_RS22105 (AAHX35_22080)	-	4579381..4580127 (+)	747	WP_044613513.1	type II secretion system protein N	-
AAHX35_RS22110 (AAHX35_22085)	pilD	4580124..4580924 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHX35_RS22115 (AAHX35_22090)	hrpB	4580971..4583400 (-)	2430	WP_374184374.1	ATP-dependent helicase HrpB	-
AAHX35_RS22120 (AAHX35_22095)	thpR	4583473..4584009 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHX35_RS22125 (AAHX35_22100)	sfsA	4584009..4584725 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHX35_RS22130 (AAHX35_22105)	dksA	4584888..4585343 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=989733 AAHX35_RS22110 WP_008807384.1 4580124..4580924(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H29_003]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=989733 AAHX35_RS22110 WP_008807384.1 4580124..4580924(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H29_003]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425