Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAHH94_RS02660 Genome accession   NZ_CP152488
Coordinates   554991..556424 (-) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain WWCOL-134     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549991..561424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHH94_RS02630 (AAHH94_02630) elbB 551566..552219 (+) 654 WP_151586562.1 isoprenoid biosynthesis glyoxalase ElbB -
  AAHH94_RS02635 (AAHH94_02635) mtgA 552216..552941 (+) 726 WP_375341679.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AAHH94_RS02640 (AAHH94_02640) npr 552938..553210 (-) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  AAHH94_RS02645 (AAHH94_02645) rapZ 553207..554061 (-) 855 WP_032614487.1 RNase adapter RapZ -
  AAHH94_RS02650 (AAHH94_02650) ptsN 554107..554598 (-) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAHH94_RS02655 (AAHH94_02655) hpf 554681..554968 (-) 288 WP_032614483.1 ribosome hibernation promoting factor -
  AAHH94_RS02660 (AAHH94_02660) rpoN/rpoN1 554991..556424 (-) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  AAHH94_RS02665 (AAHH94_02665) lptB 556472..557197 (-) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  AAHH94_RS02670 (AAHH94_02670) lptA 557204..557779 (-) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AAHH94_RS02675 (AAHH94_02675) lptC 557748..558323 (-) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  AAHH94_RS02680 (AAHH94_02680) kdsC 558320..558886 (-) 567 WP_077226309.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAHH94_RS02685 (AAHH94_02685) kdsD 558901..559887 (-) 987 WP_032614472.1 arabinose-5-phosphate isomerase KdsD -
  AAHH94_RS02690 (AAHH94_02690) - 559901..560878 (-) 978 WP_032614470.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=989676 AAHH94_RS02660 WP_032614481.1 554991..556424(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain WWCOL-134]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=989676 AAHH94_RS02660 WP_032614481.1 554991..556424(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain WWCOL-134]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGTACGCCATCCGGTACGCGCGCCGATTACCAGGATGA
TGAGTTACCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACACCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGCGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACAAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAATTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTAGAGCAACAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAACCGATGGTGCTGGCCGATATTGCCCAGGCCG
TCGAAATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCGTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment