Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BCC0238_RS16970 Genome accession   NZ_CP152478
Coordinates   3793950..3795455 (+) Length   501 a.a.
NCBI ID   WP_046577596.1    Uniprot ID   -
Organism   Burkholderia gladioli strain BCC0238     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3788950..3800455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCC0238_RS16945 (BCC0238_003273) kdsD 3790159..3791142 (+) 984 WP_025096995.1 arabinose 5-phosphate isomerase KdsD -
  BCC0238_RS16950 (BCC0238_003274) - 3791142..3791681 (+) 540 WP_013699403.1 HAD family hydrolase -
  BCC0238_RS16955 (BCC0238_003275) lptC 3791685..3792299 (+) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  BCC0238_RS16960 (BCC0238_003276) lptA 3792318..3792986 (+) 669 WP_126242734.1 lipopolysaccharide transport periplasmic protein LptA -
  BCC0238_RS16965 (BCC0238_003277) lptB 3792983..3793759 (+) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  BCC0238_RS16970 (BCC0238_003278) rpoN/rpoN1 3793950..3795455 (+) 1506 WP_046577596.1 RNA polymerase factor sigma-54 Machinery gene
  BCC0238_RS16975 (BCC0238_003279) hpf 3795606..3795971 (+) 366 WP_013699408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BCC0238_RS16980 (BCC0238_003280) - 3796185..3796697 (+) 513 WP_025097001.1 PTS sugar transporter subunit IIA -
  BCC0238_RS16985 (BCC0238_003281) hprK 3796823..3797791 (+) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  BCC0238_RS16990 (BCC0238_003282) rapZ 3797902..3798795 (+) 894 WP_013699411.1 RNase adapter RapZ -
  BCC0238_RS16995 (BCC0238_003283) - 3798828..3799463 (+) 636 WP_025097002.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55103.08 Da        Isoelectric Point: 4.9483

>NTDB_id=989658 BCC0238_RS16970 WP_046577596.1 3793950..3795455(+) (rpoN/rpoN1) [Burkholderia gladioli strain BCC0238]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEPSGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=989658 BCC0238_RS16970 WP_046577596.1 3793950..3795455(+) (rpoN/rpoN1) [Burkholderia gladioli strain BCC0238]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCCTCCTCGACGCCCGAGCCGATGCACGGT
GGCGGCTCGAACGGCAGCGAACCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGTCGCTCCTCGGGCGCCTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCACGAAGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTTACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAAGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGCGCGCGCTCCGCATCGGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGAGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACGACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCGCACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment