Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAHB66_RS20940 Genome accession   NZ_CP152474
Coordinates   4363268..4364701 (+) Length   477 a.a.
NCBI ID   WP_347114399.1    Uniprot ID   -
Organism   Leclercia sp. S52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4358268..4369701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHB66_RS20910 (AAHB66_20910) - 4358834..4359811 (+) 978 WP_347114395.1 calcium/sodium antiporter -
  AAHB66_RS20915 (AAHB66_20915) kdsD 4359825..4360812 (+) 988 Protein_4090 arabinose-5-phosphate isomerase KdsD -
  AAHB66_RS20920 (AAHB66_20920) kdsC 4360827..4361393 (+) 567 WP_333853424.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAHB66_RS20925 (AAHB66_20925) lptC 4361390..4361965 (+) 576 WP_347114396.1 LPS export ABC transporter periplasmic protein LptC -
  AAHB66_RS20930 (AAHB66_20930) lptA 4361934..4362488 (+) 555 WP_347114397.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AAHB66_RS20935 (AAHB66_20935) lptB 4362495..4363220 (+) 726 WP_250586265.1 LPS export ABC transporter ATP-binding protein -
  AAHB66_RS20940 (AAHB66_20940) rpoN/rpoN1 4363268..4364701 (+) 1434 WP_347114399.1 RNA polymerase factor sigma-54 Machinery gene
  AAHB66_RS20945 (AAHB66_20945) hpf 4364724..4365011 (+) 288 WP_106994839.1 ribosome hibernation promoting factor -
  AAHB66_RS20950 (AAHB66_20950) ptsN 4365094..4365585 (+) 492 WP_039031625.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAHB66_RS20955 (AAHB66_20955) rapZ 4365631..4366485 (+) 855 WP_250586269.1 RNase adapter RapZ -
  AAHB66_RS20960 (AAHB66_20960) npr 4366482..4366754 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  AAHB66_RS20965 (AAHB66_20965) mtgA 4366751..4367470 (-) 720 WP_347116534.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AAHB66_RS20970 (AAHB66_20970) elbB 4367473..4368126 (-) 654 WP_347114401.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54175.97 Da        Isoelectric Point: 4.3180

>NTDB_id=989603 AAHB66_RS20940 WP_347114399.1 4363268..4364701(+) (rpoN/rpoN1) [Leclercia sp. S52]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDE
ILESIGDDEIDLEEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=989603 AAHB66_RS20940 WP_347114399.1 4363268..4364701(+) (rpoN/rpoN1) [Leclercia sp. S52]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATCCGTCTGTTGCA
GCTGTCGACGCTGGAACTTCAGCAGGAGCTGCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACTCAGCAAACCCAGGACAACGAAACGCTCGACAGCGTTGACGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTTGATGCCAGTTGGGATGAGATCTACACCGCGGGCACACCGTCCGGCACGCGCGCAGACTACCAGGATGA
TGAATTGCCGGTGTATCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATCGTCGATGCCGTTGATGAGACCGGCTATTTGACCGTGTCGCTGGACGAG
ATTCTGGAAAGTATCGGTGACGATGAGATCGACCTTGAAGAGGTCGAAGCGGTTCTGAAGCGCGTCCAGCGTTTTGATCC
GGTCGGCGTCGCGGCACGGGATTTACGTGACTGCCTGCTGATCCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTTG
AAGAGGCTCGCCTGATTGTCAGCGAACATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGCGTGACGCGC
CTGAAAGAAGAGATCCTGAAAGAAGCGGTTAATCTGATCCAGTCGCTGGATCCGCGTCCGGGGCAGTCAATCCAGACCAG
CGAGCCCGAGTACGTCATTCCCGACGTGCTGGTACGCAAACATAACGGCCTGTGGACCGTGGAACTCAACTCCGACAGCA
TCCCGCGGCTGCAAATTAATCAGCAGTACGCTTCGATGTGTACCAGCTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGCAATCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGTTGCTGCGCGTCAGCCGCTGCAT
CGTGGAGCAGCAGCAGGCGTTTTTTGAACAGGGTGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCTCAGGCCG
TCGAGATGCATGAATCGACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCCTCGTCGACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.743

100

0.484

Multiple sequence alignment