Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   HMPREF9319_RS03250 Genome accession   NZ_GL397128
Coordinates   671738..673948 (+) Length   736 a.a.
NCBI ID   WP_003064161.1    Uniprot ID   E0PCU8
Organism   Streptococcus equinus ATCC 700338     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 666738..678948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMPREF9319_RS03225 (HMPREF9319_0666) miaA 667077..667964 (+) 888 WP_003064153.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  HMPREF9319_RS03230 (HMPREF9319_0667) hflX 668103..669341 (+) 1239 WP_003064155.1 GTPase HflX -
  HMPREF9319_RS03235 (HMPREF9319_0668) - 669334..669972 (+) 639 WP_003064158.1 hypothetical protein -
  HMPREF9319_RS03240 (HMPREF9319_0669) rnz 670012..670941 (+) 930 WP_003064159.1 ribonuclease Z -
  HMPREF9319_RS03245 (HMPREF9319_0670) - 670938..671696 (+) 759 WP_003064160.1 SDR family NAD(P)-dependent oxidoreductase -
  HMPREF9319_RS03250 (HMPREF9319_0671) recJ 671738..673948 (+) 2211 WP_003064161.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  HMPREF9319_RS03255 (HMPREF9319_0672) - 674367..675989 (+) 1623 Protein_652 malolactic enzyme -
  HMPREF9319_RS03260 (HMPREF9319_0673) - 676045..676989 (+) 945 WP_003064165.1 AEC family transporter -
  HMPREF9319_RS03265 (HMPREF9319_0674) bsh 677112..678092 (+) 981 WP_003064168.1 choloylglycine hydrolase -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 82594.31 Da        Isoelectric Point: 4.3917

>NTDB_id=989402 HMPREF9319_RS03250 WP_003064161.1 671738..673948(+) (recJ) [Streptococcus equinus ATCC 700338]
MITSKYNWKNIEKEPDNGFFELTKKEKLTELASQILYSRGVDTAEKLIQFLSTDLSQLHDPYLLHDMDKAVNRIRQAIEN
YEQILVYGDYDADGMTSASIMKEALEMLGAEAQVYLPNRFTDGYGPNESVYKYFIEQQNVSLIITVDNGVAGNEAIAYAQ
SQGVDVIVTDHHGLPAELPEAFAIVHPEHPEADYPFKYLAGCGVAFKVACALLESIPTEMLDLVAIGTIADMVSLTDENR
IMVKVGLEILKQTERIGLIELIKVSDVDMADVNEETVGFKIAPQLNALGRLDDPNPAVELLTGFDDEEAHNIAKMINAKN
EERKEIVQKIFDEAMSMVDLEKPVQVLAKEGWHPGVLGIVAGRILEQIAQPVIVLNIEDGIAKGSARSIEAINIFHALDE
HRDLFEAFGGHAGAAGMTLVTENLDKLSQVLCDYIAENDIDLSQKKELVIDEVLELSEINLDTVHSLEKLAPFGMDNPKP
VFEIKDFVVKQARTMGQNGAHLKLKIAQGTTAIDLVAFNQGHLAQEFQQVQNLCLATTLSINKWNGQTTVQLMLEDARVE
GVQLIDIRSKTASLPERVPVLTEDSTANEVVVLDIPDKAEELKSLFVGRHFEAIYFKNHIKRAYYLTGYGTRDQFAKLYK
TIYQFPEFDVRYKLKELSQYLNIPDILLIKMIQIFDELDFVTITDGVMVVNKDAQKRSISESQIYQDLKQQVKFQELMAL
ATPQEIYEWLINESDE

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=989402 HMPREF9319_RS03250 WP_003064161.1 671738..673948(+) (recJ) [Streptococcus equinus ATCC 700338]
ATGATAACAAGTAAATACAATTGGAAAAATATTGAAAAAGAGCCAGATAATGGCTTTTTTGAGCTGACAAAAAAAGAAAA
ACTAACAGAATTAGCTAGTCAGATTCTTTATAGTCGAGGTGTTGATACGGCAGAAAAATTGATTCAATTTTTATCAACGG
ATTTGTCACAATTGCATGACCCTTACCTTTTACATGACATGGATAAAGCGGTTAATCGTATTCGCCAAGCTATTGAGAAT
TACGAGCAAATCTTGGTTTATGGTGATTATGATGCTGACGGAATGACTAGTGCCAGCATTATGAAAGAAGCCCTTGAAAT
GTTGGGTGCCGAAGCCCAAGTTTATCTGCCAAATCGTTTCACAGATGGTTACGGCCCAAATGAATCAGTCTATAAATATT
TCATTGAGCAGCAAAACGTTTCTTTGATTATTACGGTGGACAATGGCGTAGCAGGGAACGAAGCGATTGCCTACGCACAA
AGTCAGGGGGTAGATGTCATTGTGACAGACCACCACGGTTTGCCAGCAGAGCTACCAGAAGCATTTGCGATTGTGCACCC
AGAACACCCAGAAGCTGATTATCCGTTCAAATATCTGGCTGGCTGTGGCGTTGCTTTCAAAGTTGCTTGTGCTTTGCTAG
AATCTATTCCGACAGAAATGCTTGATCTGGTGGCAATTGGAACAATTGCTGATATGGTGAGTTTGACTGATGAAAACCGC
ATTATGGTTAAAGTTGGACTTGAAATTCTGAAACAAACCGAACGCATTGGTCTGATTGAATTGATAAAAGTATCAGATGT
GGATATGGCAGATGTCAATGAAGAAACGGTTGGTTTCAAAATTGCTCCGCAGCTAAATGCACTTGGTCGCCTAGATGACC
CAAATCCTGCTGTCGAATTATTGACTGGCTTTGATGATGAAGAAGCGCATAACATTGCCAAGATGATTAATGCTAAAAAC
GAAGAGCGCAAGGAAATTGTTCAGAAAATCTTCGATGAAGCCATGAGCATGGTTGATTTGGAAAAGCCAGTGCAAGTATT
GGCTAAAGAAGGCTGGCACCCAGGCGTTTTAGGAATTGTCGCAGGGCGTATTTTAGAACAAATCGCGCAGCCAGTGATTG
TTTTAAATATCGAAGATGGGATTGCTAAAGGTTCTGCCAGAAGTATCGAGGCCATTAATATTTTCCATGCTCTTGATGAG
CACCGTGATTTATTTGAAGCCTTTGGTGGACACGCTGGCGCTGCTGGAATGACTTTGGTGACTGAAAATCTTGATAAATT
ATCACAAGTTTTGTGTGATTATATTGCTGAAAATGATATTGACTTATCACAGAAAAAAGAGCTTGTTATTGATGAGGTTC
TGGAGCTCTCAGAGATTAACCTTGATACCGTGCACAGTCTTGAAAAATTGGCACCATTTGGCATGGACAATCCAAAACCA
GTCTTTGAAATTAAAGATTTTGTGGTGAAACAAGCACGCACTATGGGGCAAAATGGTGCGCATTTAAAATTAAAAATTGC
CCAAGGTACAACGGCAATTGACCTTGTTGCCTTTAATCAAGGACATCTGGCGCAGGAATTTCAACAAGTGCAAAATCTTT
GCCTTGCAACGACATTATCGATTAATAAGTGGAATGGGCAAACGACTGTTCAATTAATGTTGGAAGATGCGCGTGTTGAG
GGTGTTCAGTTGATTGATATTCGCTCCAAAACGGCTAGCCTTCCAGAACGTGTTCCAGTATTGACTGAAGATAGCACGGC
AAATGAAGTTGTGGTGTTAGACATCCCTGATAAGGCTGAAGAATTAAAATCACTCTTTGTGGGTCGTCATTTTGAAGCCA
TTTATTTTAAAAATCATATTAAACGTGCTTATTATCTGACAGGCTATGGCACACGTGACCAATTTGCTAAGCTTTATAAA
ACCATTTATCAATTCCCAGAATTTGATGTCCGCTACAAGTTGAAAGAATTGAGCCAATACCTTAATATCCCTGATATTTT
GCTCATTAAAATGATTCAGATTTTTGATGAATTAGATTTTGTAACCATCACAGATGGTGTTATGGTCGTCAACAAAGATG
CTCAGAAACGCAGTATTTCAGAAAGCCAAATCTACCAAGACCTCAAACAACAAGTTAAATTCCAAGAATTGATGGCACTA
GCTACTCCCCAAGAAATATATGAGTGGTTGATTAATGAAAGTGATGAGTAG

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(583-730)

RecJ OB domain (RecJ_OB)

(450-556)

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0PCU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

67.164

100

0.673
  recJ Bacillus subtilis subsp. subtilis str. 168

39.226

100

0.413