Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V7S45_RS19615 Genome accession   NZ_CP152433
Coordinates   3846695..3847129 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus sp. BR3(2024)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3841695..3852129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7S45_RS19600 (V7S45_19605) - 3842871..3843428 (+) 558 WP_089607607.1 PadR family transcriptional regulator -
  V7S45_RS19605 (V7S45_19610) metE 3843942..3846230 (+) 2289 WP_088343883.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  V7S45_RS19610 (V7S45_19615) comJ 3846269..3846658 (-) 390 WP_000424030.1 competence protein ComJ -
  V7S45_RS19615 (V7S45_19620) nucA/comI 3846695..3847129 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  V7S45_RS19620 (V7S45_19625) - 3847356..3848105 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  V7S45_RS19625 (V7S45_19630) kinB 3848144..3849418 (-) 1275 WP_088343885.1 sporulation sensor histidine kinase KinB -
  V7S45_RS19630 (V7S45_19635) - 3849729..3849929 (+) 201 WP_000929278.1 hypothetical protein -
  V7S45_RS19635 (V7S45_19640) - 3850053..3851216 (+) 1164 WP_000434582.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=989377 V7S45_RS19615 WP_000811494.1 3846695..3847129(-) (nucA/comI) [Bacillus sp. BR3(2024)]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=989377 V7S45_RS19615 WP_000811494.1 3846695..3847129(-) (nucA/comI) [Bacillus sp. BR3(2024)]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCAAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment