Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AAG068_RS21585 Genome accession   NZ_CP152427
Coordinates   4171687..4172433 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus paramycoides strain JYZ-SD5     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4166687..4177433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG068_RS21560 (AAG068_21560) dnaG 4167455..4169251 (-) 1797 WP_000528216.1 DNA primase -
  AAG068_RS21565 (AAG068_21565) - 4169582..4169737 (-) 156 WP_342715761.1 hypothetical protein -
  AAG068_RS21570 (AAG068_21570) - 4169926..4170069 (-) 144 WP_342719829.1 hypothetical protein -
  AAG068_RS21575 (AAG068_21575) - 4170105..4170917 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  AAG068_RS21580 (AAG068_21580) - 4170947..4171579 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  AAG068_RS21585 (AAG068_21585) recO 4171687..4172433 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  AAG068_RS21590 (AAG068_21590) - 4172467..4172610 (-) 144 WP_000883923.1 YqzL family protein -
  AAG068_RS21595 (AAG068_21595) era 4172787..4173692 (-) 906 WP_001080241.1 GTPase Era -
  AAG068_RS21600 (AAG068_21600) - 4173685..4174083 (-) 399 WP_001086294.1 cytidine deaminase -
  AAG068_RS21605 (AAG068_21605) - 4174183..4174536 (-) 354 WP_342715762.1 diacylglycerol kinase family protein -
  AAG068_RS21610 (AAG068_21610) ybeY 4174533..4175003 (-) 471 WP_342715763.1 rRNA maturation RNase YbeY -
  AAG068_RS21615 (AAG068_21615) - 4175000..4177144 (-) 2145 WP_342715764.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=989331 AAG068_RS21585 WP_000487010.1 4171687..4172433(-) (recO) [Bacillus paramycoides strain JYZ-SD5]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=989331 AAG068_RS21585 WP_000487010.1 4171687..4172433(-) (recO) [Bacillus paramycoides strain JYZ-SD5]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAATTGGGTAAGGTAAGTGCGATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGGCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACGGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCAGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCAGGGAAGGCGGTTTTCTGTGCTCGCGTCATGCTGAGCAAGACCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTACGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGTA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment