Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAG068_RS20090 Genome accession   NZ_CP152427
Coordinates   3917228..3917662 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus paramycoides strain JYZ-SD5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3912228..3922662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG068_RS20075 (AAG068_20075) - 3913252..3913809 (+) 558 WP_342715590.1 PadR family transcriptional regulator -
  AAG068_RS20080 (AAG068_20080) metE 3914477..3916765 (+) 2289 WP_342715591.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AAG068_RS20085 (AAG068_20085) comJ 3916803..3917192 (-) 390 WP_074606329.1 competence protein ComJ -
  AAG068_RS20090 (AAG068_20090) nucA/comI 3917228..3917662 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  AAG068_RS20095 (AAG068_20095) - 3917899..3918648 (+) 750 WP_342715592.1 DUF3967 domain-containing protein -
  AAG068_RS20100 (AAG068_20100) kinB 3918696..3919970 (-) 1275 WP_342715593.1 sporulation sensor histidine kinase KinB -
  AAG068_RS20105 (AAG068_20105) - 3920279..3920479 (+) 201 WP_000929281.1 hypothetical protein -
  AAG068_RS20110 (AAG068_20110) - 3920602..3921765 (+) 1164 WP_342715594.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=989327 AAG068_RS20090 WP_000811502.1 3917228..3917662(-) (nucA/comI) [Bacillus paramycoides strain JYZ-SD5]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=989327 AAG068_RS20090 WP_000811502.1 3917228..3917662(-) (nucA/comI) [Bacillus paramycoides strain JYZ-SD5]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGAA
AACATATTACGGATGCGATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGATATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAGTTTGATGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment