Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAG068_RS00530 Genome accession   NZ_CP152427
Coordinates   97790..99166 (+) Length   458 a.a.
NCBI ID   WP_001085207.1    Uniprot ID   A0A2B4FB19
Organism   Bacillus paramycoides strain JYZ-SD5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92790..104166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG068_RS00510 (AAG068_00510) ctsR 92983..93444 (+) 462 WP_342716605.1 transcriptional regulator CtsR -
  AAG068_RS00515 (AAG068_00515) - 93618..94166 (+) 549 WP_342716606.1 UvrB/UvrC motif-containing protein -
  AAG068_RS00520 (AAG068_00520) - 94171..95235 (+) 1065 WP_342716607.1 protein arginine kinase -
  AAG068_RS00525 (AAG068_00525) clpC 95258..97693 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  AAG068_RS00530 (AAG068_00530) radA 97790..99166 (+) 1377 WP_001085207.1 DNA repair protein RadA Machinery gene
  AAG068_RS00535 (AAG068_00535) disA 99170..100243 (+) 1074 WP_342716608.1 DNA integrity scanning diadenylate cyclase DisA -
  AAG068_RS00540 (AAG068_00540) - 100404..101513 (+) 1110 WP_342716609.1 PIN/TRAM domain-containing protein -
  AAG068_RS00545 (AAG068_00545) ispD 101530..102210 (+) 681 WP_342716610.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AAG068_RS00550 (AAG068_00550) ispF 102326..102802 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49814.39 Da        Isoelectric Point: 7.1421

>NTDB_id=989305 AAG068_RS00530 WP_001085207.1 97790..99166(+) (radA) [Bacillus paramycoides strain JYZ-SD5]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=989305 AAG068_RS00530 WP_001085207.1 97790..99166(+) (radA) [Bacillus paramycoides strain JYZ-SD5]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCGAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAATTT
AATCGTGTACTTGGTGGCGGGATTGTAGATGGTTCCTTAGTACTTATTGGCGGAGACCCTGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGCGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
GCACACATTGAAGAAGTGAATCCAGCCTTTGTTGTTATTGATTCCATCCAAACGATACATTTACCTGAGGTAACGTCAGC
ACCGGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGGATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGCGCAATCGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATATCGTATTTTGCGAGCTGTGAAGAATCGTTTTGGTTCTACAAATGAAATGGGTATTTT
TGAAATGAAGGAACTTGGTCTTGCAGAAGTCTTAAACCCTTCTGAAATTTTCCTGGAGGAAAGACCAGTTGGGGTTGCGG
GATCGACAGTGGTTGCCTCAATGGAAGGAACACGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGACCATAACCGTGTATCGCTTATTATGGCAGTGTTAGAAAAAAGAACAGG
TTTATTATTGCAAAATCAAGACGCATATTTAAAAGTAGCAGGCGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCGACCGATGCAGTAATAGGAGAAGTGGGATTAACT
GGAGAAATAAGAAGGGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTAGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCCAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B4FB19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629

Multiple sequence alignment