Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAGF34_RS02700 Genome accession   NZ_CP152376
Coordinates   583961..585556 (-) Length   531 a.a.
NCBI ID   WP_342619094.1    Uniprot ID   -
Organism   Rhodoferax sp. GW822-FHT02A01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 578961..590556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGF34_RS02675 (AAGF34_02675) - 579109..579714 (-) 606 WP_342619089.1 thioredoxin fold domain-containing protein -
  AAGF34_RS02680 (AAGF34_02680) - 579742..580584 (-) 843 WP_342619090.1 UPF0149 family protein -
  AAGF34_RS02685 (AAGF34_02685) - 580638..582287 (+) 1650 WP_342619091.1 FMN-binding glutamate synthase family protein -
  AAGF34_RS02690 (AAGF34_02690) - 582247..582693 (-) 447 WP_342619092.1 putative toxin-antitoxin system toxin component, PIN family -
  AAGF34_RS02695 (AAGF34_02695) - 582693..583964 (-) 1272 WP_342619093.1 class I SAM-dependent RNA methyltransferase -
  AAGF34_RS02700 (AAGF34_02700) rpoN/rpoN1 583961..585556 (-) 1596 WP_342619094.1 RNA polymerase factor sigma-54 Machinery gene
  AAGF34_RS02705 (AAGF34_02705) lptB 585556..586311 (-) 756 WP_342619095.1 LPS export ABC transporter ATP-binding protein -
  AAGF34_RS02710 (AAGF34_02710) lptA 586308..586928 (-) 621 WP_342619096.1 lipopolysaccharide transport periplasmic protein LptA -
  AAGF34_RS02715 (AAGF34_02715) - 587037..587966 (-) 930 WP_342619097.1 exopolyphosphatase -
  AAGF34_RS02720 (AAGF34_02720) - 588063..588716 (-) 654 WP_342619098.1 thiol:disulfide interchange protein DsbA/DsbL -
  AAGF34_RS02725 (AAGF34_02725) - 588788..589453 (-) 666 WP_342619099.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 58898.50 Da        Isoelectric Point: 4.6594

>NTDB_id=988737 AAGF34_RS02700 WP_342619094.1 583961..585556(-) (rpoN/rpoN1) [Rhodoferax sp. GW822-FHT02A01]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLELGEDHAEREEFGIAQADTPVSEGDKDVEFSAT
MPDLGSSTVASTSSDEDNSPLSGDEGIDWEGDGTTEMTPDDGEWGVEAKASTNNLTGDEDKDATELARTQESLQAFLHRQ
TLGLRLSAEDAAALRFLIESLNDDGYLEEPLEDLARTLNPQDEEQLYDLVHHFSVALGLLQSLEPVGVGARNLSECLSLQ
LKALLRNPDLEDEEREQLEVALRICQQPMELLARRDIKRLVQLGVGSDALVREAIAQIARLEPKPGRRFIDVERNIVVPD
VLVVPVTNANKAAAPKFRVMLNPDVMPRLKVHDIYANVLRNHRGGKDSASHAALQQRLQEARWFIKNIQQRFDTILRVST
AIVERQKSFFTHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTFELKYFFGSGLGTESGGNASSTAVRALIK
QFISAESAKKPLSDNQISEMLKEQGIECARRTVAKYREGLKIAPASLRKAL

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=988737 AAGF34_RS02700 WP_342619094.1 583961..585556(-) (rpoN/rpoN1) [Rhodoferax sp. GW822-FHT02A01]
ATGAAGCAAGGTCTCTCATTACGCGTCTCCCAGCATCTGGCGCTCACGCCCCAGTTGCAGCAGTCGATCCGTCTTCTGCA
GCTCTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTGGACGAGAACCCTTTTCTGGAACTGGGTGAGGACCATG
CCGAGCGGGAAGAGTTCGGCATCGCACAGGCGGATACGCCGGTCAGTGAAGGCGACAAGGATGTGGAGTTTTCCGCCACC
ATGCCCGACCTGGGCAGCAGCACCGTGGCCTCCACCAGCAGTGATGAAGACAACAGTCCCCTGAGCGGGGACGAAGGCAT
CGACTGGGAAGGCGACGGCACCACCGAAATGACCCCGGACGACGGCGAGTGGGGCGTCGAGGCCAAGGCCAGTACCAATA
ACCTCACTGGCGACGAAGACAAGGACGCCACCGAGCTGGCCCGCACGCAGGAGAGCTTGCAGGCCTTCCTGCATCGGCAG
ACACTGGGTTTGCGCCTGAGCGCCGAAGACGCTGCCGCGCTGCGTTTCCTGATCGAGTCGCTCAACGATGACGGCTATCT
GGAAGAGCCGCTCGAGGACCTGGCCCGCACCTTGAACCCGCAGGATGAAGAGCAGTTGTACGACCTGGTGCACCATTTCT
CGGTAGCCCTGGGACTGCTGCAAAGCCTGGAGCCGGTTGGCGTCGGTGCCCGCAATTTGAGCGAGTGCCTCAGTCTGCAA
TTGAAAGCTCTGCTGCGCAATCCGGACCTGGAAGACGAAGAGCGCGAGCAACTGGAAGTGGCCCTGCGCATTTGCCAGCA
GCCCATGGAATTGCTGGCGCGCCGTGACATCAAGCGGCTGGTGCAATTGGGCGTGGGCAGCGATGCCCTGGTGCGCGAGG
CCATAGCGCAGATTGCGCGGCTGGAACCCAAGCCGGGGCGGCGCTTTATCGACGTGGAGCGCAACATCGTGGTGCCCGAC
GTGCTGGTGGTGCCGGTGACCAACGCCAACAAGGCGGCTGCACCCAAGTTCCGCGTCATGCTCAATCCCGATGTGATGCC
GCGCCTCAAGGTGCATGACATCTACGCCAATGTGCTGCGCAACCACCGCGGTGGCAAGGACAGCGCCAGCCACGCCGCGC
TGCAGCAACGGCTGCAGGAGGCGCGTTGGTTCATCAAGAACATCCAGCAGCGCTTTGACACCATCCTGCGCGTGAGCACG
GCGATTGTGGAGCGGCAGAAGAGCTTCTTCACCCATGGCGAATTGGCCATGCGCCCGCTGGTGCTGCGTGAGATCGCCGA
CGAACTGGGCTTGCACGAGTCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCAACGCCTTTTGGCACCTTTGAGC
TGAAGTACTTCTTCGGCTCCGGCCTGGGCACTGAGTCCGGCGGCAATGCGTCCAGCACCGCGGTGCGTGCACTCATCAAG
CAGTTCATCAGCGCCGAAAGTGCCAAGAAGCCGCTGTCAGACAACCAGATATCCGAGATGCTCAAGGAGCAGGGTATTGA
ATGTGCCCGGCGCACTGTGGCCAAATACCGCGAGGGGCTGAAGATCGCCCCCGCATCCCTGCGCAAGGCGCTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-355)

Sigma-54, DNA binding domain (Sigma54_DBD)

(373-529)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.813

100

0.501

Multiple sequence alignment