Detailed information NTG0988698

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: AAFR80_RS12775
• Genome accession: NZ_CP152371
• Coordinates: 196721..197881 (-)
• Length: 386 a.a.
• NCBI ID: WP_011657978.1
• Uniprot ID: Q0BBP8
• Organism: Burkholderia ambifaria strain CF3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 191721..202881

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFR80_RS12755 (AAFR80_12755)	-	192240..192623 (+)	384	WP_165752060.1	nuclear transport factor 2 family protein	-
AAFR80_RS12760 (AAFR80_12760)	leuA	192767..194413 (-)	1647	WP_212070365.1	2-isopropylmalate synthase	-
AAFR80_RS12765 (AAFR80_12765)	ybiB	194804..195778 (-)	975	WP_175699744.1	DNA-binding protein YbiB	-
AAFR80_RS12770 (AAFR80_12770)	maiA	195968..196612 (+)	645	WP_006760402.1	maleylacetoacetate isomerase	-
AAFR80_RS12775 (AAFR80_12775)	pilA	196721..197881 (-)	1161	WP_011657978.1	signal recognition particle-docking protein FtsY	Machinery gene
AAFR80_RS12780 (AAFR80_12780)	rsmD	198083..198697 (+)	615	WP_175699745.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
AAFR80_RS12785 (AAFR80_12785)	coaD	198809..199306 (+)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
AAFR80_RS12790 (AAFR80_12790)	-	199366..199632 (+)	267	WP_011657976.1	YfhL family 4Fe-4S dicluster ferredoxin	-
AAFR80_RS12795 (AAFR80_12795)	hisC	199738..200805 (-)	1068	WP_342612800.1	histidinol-phosphate transaminase	-
AAFR80_RS12800 (AAFR80_12800)	pth	200867..201466 (-)	600	WP_006760396.1	aminoacyl-tRNA hydrolase	-
AAFR80_RS12805 (AAFR80_12805)	-	201596..202204 (-)	609	WP_006753282.1	50S ribosomal protein L25/general stress protein Ctc	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 41170.99 Da
• Isoelectric Point: 4.8019

>NTDB_id=988698 AAFR80_RS12775 WP_011657978.1 196721..197881(-) (pilA) [Burkholderia ambifaria strain CF3]
MFSFFKRFKKTQESDSTESQSADALQPDEPSDAPAADARQAIEAPPAPAQPQPAAPAVVMTVTPTNDGSDDVVEAVEIVP
PPTQDASAKKSWLTRLKTGLAKTGSSITGVFVNTKIDEDLYEELEAALLMSDAGVDATEYLLGALREKVKTGRLTDPQQV
KSALHDLLVELLKPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAVEFADALFD

Nucleotide

• Length: 1161 bp

>NTDB_id=988698 AAFR80_RS12775 WP_011657978.1 196721..197881(-) (pilA) [Burkholderia ambifaria strain CF3]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGTCCGACTCCACGGAATCGCAATCGGCCGACGCGCTGCAACC
GGACGAGCCGTCCGACGCGCCCGCCGCCGATGCCCGTCAGGCGATCGAAGCCCCGCCAGCCCCCGCGCAACCGCAACCGG
CCGCGCCGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGCAGCGACGACGTCGTCGAAGCGGTCGAGATCGTGCCG
CCGCCGACGCAGGACGCCTCCGCGAAGAAATCGTGGCTCACCCGCCTGAAAACGGGGCTCGCGAAGACGGGTTCGAGCAT
TACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAGGAACTCGAAGCCGCGCTGCTGATGTCCGACGCCG
GCGTCGATGCGACCGAGTACCTGCTCGGTGCGCTGCGCGAGAAAGTGAAAACGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGAGCGCGCTGCACGATCTGCTCGTCGAACTGCTGAAGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACGGGCGTGAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCG
ACCAGTCGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTCCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGTGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCTCGAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGATGCGCTCGGCCTCACGGGCCTGATCGTCACGAAACTCGACGGCACGGCCAAGGGCGG
GATTCTCGCCGCGATCGCGCGGCAGCGCCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAAAAGGTCGAGGACCTGCAGC
CGTTCAACGCCGTCGAATTCGCGGACGCGCTATTCGATTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-385)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: Q0BBP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.632	100	0.57