Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WN937_RS14875 Genome accession   NZ_CP152363
Coordinates   3132589..3133605 (-) Length   338 a.a.
NCBI ID   WP_031821574.1    Uniprot ID   A0AA47L644
Organism   Vibrio parahaemolyticus strain vp-HL-201910     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3127589..3138605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN937_RS14850 (WN937_14850) aroK 3128449..3128967 (-) 519 WP_005381319.1 shikimate kinase AroK -
  WN937_RS14855 (WN937_14855) pilQ 3129156..3130904 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  WN937_RS14860 (WN937_14860) pilP 3130936..3131451 (-) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  WN937_RS14865 (WN937_14865) pilO 3131441..3132034 (-) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  WN937_RS14870 (WN937_14870) pilN 3132027..3132605 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  WN937_RS14875 (WN937_14875) pilM 3132589..3133605 (-) 1017 WP_031821574.1 type IV pilus assembly protein PilM Machinery gene
  WN937_RS14880 (WN937_14880) - 3133748..3136318 (+) 2571 WP_069535787.1 PBP1A family penicillin-binding protein -
  WN937_RS14885 (WN937_14885) oxyR 3136374..3137282 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  WN937_RS14890 (WN937_14890) - 3137453..3138181 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37720.16 Da        Isoelectric Point: 6.6631

>NTDB_id=988687 WN937_RS14875 WP_031821574.1 3132589..3133605(-) (pilM) [Vibrio parahaemolyticus strain vp-HL-201910]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWNLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=988687 WN937_RS14875 WP_031821574.1 3132589..3133605(-) (pilM) [Vibrio parahaemolyticus strain vp-HL-201910]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAATCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGAACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.32

100

0.843
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment