Detailed information NTG0987132

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AAGT87_RS14580
• Genome accession: NZ_CP152071
• Coordinates: 2155773..2156789 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae strain Sudan_2019_18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2150773..2161789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAGT87_RS14565 (AAGT87_14570)	-	2151315..2152046 (-)	732	WP_000495223.1	glutathione peroxidase	-
AAGT87_RS14570 (AAGT87_14575)	oxyR	2152186..2153079 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
AAGT87_RS14575 (AAGT87_14580)	-	2153153..2155630 (-)	2478	WP_353495223.1	penicillin-binding protein 1A	-
AAGT87_RS14580 (AAGT87_14585)	pilM	2155773..2156789 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
AAGT87_RS14585 (AAGT87_14590)	pilN	2156779..2157363 (+)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
AAGT87_RS14590 (AAGT87_14595)	pilO	2157356..2157946 (+)	591	WP_353495224.1	type 4a pilus biogenesis protein PilO	Machinery gene
AAGT87_RS14595 (AAGT87_14600)	pilP	2157936..2158454 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
AAGT87_RS14600 (AAGT87_14605)	pilQ	2158480..2160195 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ family protein	Machinery gene
AAGT87_RS14605 (AAGT87_14610)	aroK	2160382..2160906 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=987132 AAGT87_RS14580 WP_000520444.1 2155773..2156789(+) (pilM) [Vibrio cholerae strain Sudan_2019_18]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=987132 AAGT87_RS14580 WP_000520444.1 2155773..2156789(+) (pilM) [Vibrio cholerae strain Sudan_2019_18]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCTGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485