Detailed information NTG0986947

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: MHB67_RS16250
• Genome accession: NZ_CP152051
• Coordinates: 3184166..3185563 (-)
• Length: 465 a.a.
• NCBI ID: WP_279586125.1
• Uniprot ID: -
• Organism: Bacillus sp. FSL H8-0516
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3179166..3190563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MHB67_RS16215 (MHB67_16215)	flgL	3179367..3180275 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
MHB67_RS16220 (MHB67_16220)	flgK	3180285..3181805 (-)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
MHB67_RS16225 (MHB67_16225)	-	3181821..3182303 (-)	483	WP_024423619.1	flagellar protein FlgN	-
MHB67_RS16230 (MHB67_16230)	flgM	3182322..3182585 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
MHB67_RS16235 (MHB67_16235)	-	3182665..3183084 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
MHB67_RS16240 (MHB67_16240)	comFC	3183141..3183797 (-)	657	WP_243837412.1	ComF family protein	Machinery gene
MHB67_RS16245 (MHB67_16245)	-	3183824..3184117 (-)	294	WP_024423623.1	competence protein ComFB	-
MHB67_RS16250 (MHB67_16250)	comFA	3184166..3185563 (-)	1398	WP_279586125.1	DEAD/DEAH box helicase	Machinery gene
MHB67_RS16255 (MHB67_16255)	-	3185684..3186526 (-)	843	WP_024423625.1	DegV family protein	-
MHB67_RS16260 (MHB67_16260)	degU	3186742..3187431 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
MHB67_RS16265 (MHB67_16265)	degS	3187496..3188671 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator
MHB67_RS16270 (MHB67_16270)	-	3188893..3189561 (+)	669	WP_012011434.1	YigZ family protein	-

Sequence

Protein

• Length: 465 a.a.
• Molecular weight: 53252.21 Da
• Isoelectric Point: 9.8999

>NTDB_id=986947 MHB67_RS16250 WP_279586125.1 3184166..3185563(-) (comFA) [Bacillus sp. FSL H8-0516]
MNMDKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKVNGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC
RSCIMMGKVAECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMNVCIATPRTDVVLELEPRLRKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMQ
RHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide

• Length: 1398 bp

>NTDB_id=986947 MHB67_RS16250 WP_279586125.1 3184166..3185563(-) (comFA) [Bacillus sp. FSL H8-0516]
TTGAATATGGACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGTAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGATTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGTAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTACTTTTTCACGGAATCGAATATGCGTTA
AATCAAGGAATGAATGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTAGAAAAGCATT
TCAAGGGGTGACAATTACCGTACTTTACGGAGGTAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTCAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC
CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG
AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG
AGAACAATCCTTGAACGTGGAGGGGGTATCTGCCGATGATCCGGATAGAAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCGAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGGCGGGTAGGCAGACACCCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (116-265)

• Helicase conserved C-terminal domain (Helicase_C) (324-420)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.412	98.065	0.514
comFA/cflA	Streptococcus pneumoniae Rx1	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae D39	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae R6	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae TIGR4	38.269	94.409	0.361