Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHB67_RS01890 Genome accession   NZ_CP152051
Coordinates   352121..352549 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. FSL H8-0516     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 347121..357549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB67_RS01860 (MHB67_01860) - 347285..347614 (+) 330 WP_024424311.1 YckD family protein -
  MHB67_RS01865 (MHB67_01865) - 347658..348422 (-) 765 WP_213404990.1 MBL fold metallo-hydrolase -
  MHB67_RS01870 (MHB67_01870) - 348538..348954 (+) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  MHB67_RS01875 (MHB67_01875) - 348983..349789 (+) 807 WP_024424308.1 AAC(3) family N-acetyltransferase -
  MHB67_RS01880 (MHB67_01880) - 349791..350177 (-) 387 WP_024424307.1 competence protein ComJ -
  MHB67_RS01885 (MHB67_01885) - 350296..351993 (-) 1698 WP_342492745.1 methyl-accepting chemotaxis protein -
  MHB67_RS01890 (MHB67_01890) nucA/comI 352121..352549 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHB67_RS01895 (MHB67_01895) - 352917..354986 (+) 2070 WP_024427271.1 hydantoinase/oxoprolinase family protein -
  MHB67_RS01900 (MHB67_01900) - 354976..356964 (+) 1989 WP_044332682.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=986901 MHB67_RS01890 WP_024424305.1 352121..352549(-) (nucA/comI) [Bacillus sp. FSL H8-0516]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=986901 MHB67_RS01890 WP_024424305.1 352121..352549(-) (nucA/comI) [Bacillus sp. FSL H8-0516]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment