Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHI16_RS00590 Genome accession   NZ_CP152044
Coordinates   109202..110581 (+) Length   459 a.a.
NCBI ID   WP_058015795.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0923     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104202..115581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI16_RS00570 (MHI16_00570) - 104544..105008 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  MHI16_RS00575 (MHI16_00575) - 105023..105580 (+) 558 WP_034665655.1 UvrB/UvrC motif-containing protein -
  MHI16_RS00580 (MHI16_00580) - 105585..106676 (+) 1092 WP_058015794.1 protein arginine kinase -
  MHI16_RS00585 (MHI16_00585) clpC 106673..109108 (+) 2436 WP_003216969.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MHI16_RS00590 (MHI16_00590) radA 109202..110581 (+) 1380 WP_058015795.1 DNA repair protein RadA Machinery gene
  MHI16_RS00595 (MHI16_00595) disA 110584..111663 (+) 1080 WP_012008686.1 DNA integrity scanning diadenylate cyclase DisA -
  MHI16_RS00600 (MHI16_00600) - 111817..112917 (+) 1101 WP_058015822.1 PIN/TRAM domain-containing protein -
  MHI16_RS00605 (MHI16_00605) ispD 112931..113620 (+) 690 WP_058015796.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHI16_RS00610 (MHI16_00610) ispF 113624..114100 (+) 477 WP_058015797.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50052.60 Da        Isoelectric Point: 7.9305

>NTDB_id=986581 MHI16_RS00590 WP_058015795.1 109202..110581(+) (radA) [Bacillus sp. FSL K6-0923]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPVNRRSAFNHSVQTIQKPSPISAIETSDEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIKSTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=986581 MHI16_RS00590 WP_058015795.1 109202..110581(+) (radA) [Bacillus sp. FSL K6-0923]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAATCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCGGTAAACCGTCGAAGCGCTTTTAATCATTCTGTCC
AAACCATTCAAAAACCTTCACCTATTTCTGCAATTGAAACATCAGATGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGAATTGGGAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTATTATACATATCCGGTGAGGAGTCCATTAAACAAA
CGAAGTTAAGAGCGGACCGCCTCGGCATAAAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTCGTGGTGGATTCGATTCAAACTGTTTATCAAAGCGATATTACGTCAGC
TCCTGGGTCTGTATCTCAAGTGAGAGAATGTACAGCGCAGCTGATGAAGATTGCCAAGACAAATGGGATTCCAATTTTTA
TTGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGTCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGTGAGCGTCATCATACGTTTCGTATCTTACGTGCAGTGAAAAACCGATTTGGTTCAACGAATGAACTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCCGAAATTTTCTTAGAAGAACGTTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTTGTCGAGATACAAGCGCTAATTTCACCAACAAGCTTC
GGAAATCCGCGTAGAATGGCCACTGGTCTTGATCATAATCGAGTGTCACTGCTCATGGCGGTTTTAGAAAAACGTGTTGG
ACTGCTGCTGCAAAACCAGGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCAATTGACTTGGCCA
TTGCGGTCAGTATTGCCTCCAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCGAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

86.9

99.782

0.867
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment