Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHB69_RS01955 Genome accession   NZ_CP152043
Coordinates   359763..360191 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. FSL K6-0994     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 354763..365191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB69_RS01925 (MHB69_01925) - 354925..355254 (+) 330 WP_342496224.1 YckD family protein -
  MHB69_RS01930 (MHB69_01930) - 355298..356062 (-) 765 WP_034283296.1 MBL fold metallo-hydrolase -
  MHB69_RS01935 (MHB69_01935) - 356178..356594 (+) 417 WP_342495937.1 Lrp/AsnC family transcriptional regulator -
  MHB69_RS01940 (MHB69_01940) - 356623..357429 (+) 807 WP_101677032.1 AAC(3) family N-acetyltransferase -
  MHB69_RS01945 (MHB69_01945) - 357431..357817 (-) 387 WP_342495938.1 competence protein ComJ -
  MHB69_RS01950 (MHB69_01950) - 357938..359635 (-) 1698 WP_046313811.1 methyl-accepting chemotaxis protein -
  MHB69_RS01955 (MHB69_01955) nucA/comI 359763..360191 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHB69_RS01960 (MHB69_01960) - 360559..362628 (+) 2070 WP_342495939.1 hydantoinase/oxoprolinase family protein -
  MHB69_RS01965 (MHB69_01965) - 362618..364606 (+) 1989 WP_342495940.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=986522 MHB69_RS01955 WP_024424305.1 359763..360191(-) (nucA/comI) [Bacillus sp. FSL K6-0994]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=986522 MHB69_RS01955 WP_024424305.1 359763..360191(-) (nucA/comI) [Bacillus sp. FSL K6-0994]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCGATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCAGACG
GCACAAGAATCCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment