Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI46_RS08805 Genome accession   NZ_CP152042
Coordinates   1741735..1742115 (+) Length   126 a.a.
NCBI ID   WP_228280000.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1000     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1736735..1747115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS08795 (MHI46_08795) - 1737229..1740372 (+) 3144 WP_260775973.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  MHI46_RS08800 (MHI46_08800) - 1740567..1741475 (+) 909 WP_144491261.1 trypsin-like serine protease -
  MHI46_RS08805 (MHI46_08805) nucA/comI 1741735..1742115 (+) 381 WP_228280000.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI46_RS08810 (MHI46_08810) - 1742263..1743111 (+) 849 WP_039178899.1 STAS domain-containing protein -
  MHI46_RS08815 (MHI46_08815) - 1743144..1743686 (-) 543 WP_039178902.1 IseA DL-endopeptidase inhibitor family protein -
  MHI46_RS08820 (MHI46_08820) - 1743984..1744430 (+) 447 WP_039178904.1 hypothetical protein -
  MHI46_RS08825 (MHI46_08825) lexA 1744476..1745096 (-) 621 WP_024425129.1 transcriptional repressor LexA -
  MHI46_RS08830 (MHI46_08830) yneA 1745255..1745566 (+) 312 WP_034283425.1 cell division suppressor protein YneA -
  MHI46_RS08835 (MHI46_08835) - 1745584..1746231 (+) 648 WP_342503226.1 recombinase family protein -
  MHI46_RS08840 (MHI46_08840) - 1746301..1746531 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13757.50 Da        Isoelectric Point: 8.5068

>NTDB_id=986486 MHI46_RS08805 WP_228280000.1 1741735..1742115(+) (nucA/comI) [Bacillus sp. FSL K6-1000]
MGMLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=986486 MHI46_RS08805 WP_228280000.1 1741735..1742115(+) (nucA/comI) [Bacillus sp. FSL K6-1000]
ATGGGCATGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACA
GGAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGGATTCCGACAAAACCTGGATTTGACCGGGATGAATGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGATTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.349

100

0.563

Multiple sequence alignment