Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NYE62_RS05385 Genome accession   NZ_CP152037
Coordinates   1080458..1084162 (+) Length   1234 a.a.
NCBI ID   WP_251257174.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2861     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1075458..1089162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE62_RS05370 (NYE62_05370) - 1075538..1076047 (-) 510 WP_015417176.1 ferritin-like domain-containing protein -
  NYE62_RS05375 (NYE62_05375) - 1076116..1076829 (-) 714 WP_144664071.1 DUF421 domain-containing protein -
  NYE62_RS05380 (NYE62_05380) addB 1076971..1080471 (+) 3501 WP_342497281.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NYE62_RS05385 (NYE62_05385) addA 1080458..1084162 (+) 3705 WP_251257174.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NYE62_RS05390 (NYE62_05390) sbcD 1084227..1085399 (+) 1173 WP_007409169.1 exonuclease subunit SbcD -
  NYE62_RS05395 (NYE62_05395) sbcC 1085396..1088788 (+) 3393 WP_342497282.1 exonuclease subunit SbcC -
  NYE62_RS05400 (NYE62_05400) - 1088804..1089097 (+) 294 WP_003155144.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141179.48 Da        Isoelectric Point: 5.1554

>NTDB_id=986194 NYE62_RS05385 WP_251257174.1 1080458..1084162(+) (addA) [Bacillus sp. FSL K6-2861]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELPFYQYVREDAEMALYGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLNEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSE
LLEENPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGK
DLLGALRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=986194 NYE62_RS05385 WP_251257174.1 1080458..1084162(+) (addA) [Bacillus sp. FSL K6-2861]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTTT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CGGTTGATGTTGACCGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCAGACAGATACACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAAAGCTTTGTCCGCCTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAATATGTGAGAGAAGATGCTGAAATGGCGCTCTACGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCCTTTAAGCGGGCGAAGGCGGTAAAGG
GCGATGAATTTGACAAAGCGCTTTTGAACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACCGATTACTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAACAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGAGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACGAATCTTGTGCAAGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTTAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGACCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGATTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACTGTGCATTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCGGGTTACTTTGAAGCCGTGGAGGTAGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGATCGCCCATCGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGCTGACAGGCATGATGACCTGTATCAAAAACTGAGAACCT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGACTATGCAGGCGGCATGCCAGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCCCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAA
AGATGCGCAGAGAGCTCTTATCCGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTTCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACA
GGACGCCGGGACTTTCCTCATCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCATATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGAGAATCCGAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGAGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGAAGCTATTGATATAGAAGAAATTGTCCAGTTTTTCGGAACTGAAATCGGGAAA
GACCTTCTCGGGGCGCTGCGGATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACTGCGGACGGTCTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.334

100

0.823

Multiple sequence alignment