Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MHI58_RS12350 Genome accession   NZ_CP152035
Coordinates   2390110..2390880 (-) Length   256 a.a.
NCBI ID   WP_007501304.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2869     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2385110..2395880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI58_RS12330 (MHI58_12330) - 2385203..2386024 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  MHI58_RS12335 (MHI58_12335) - 2386050..2386688 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  MHI58_RS12340 (MHI58_12340) glyS 2386855..2388900 (-) 2046 WP_045034385.1 glycine--tRNA ligase subunit beta -
  MHI58_RS12345 (MHI58_12345) glyQ 2388893..2389780 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  MHI58_RS12350 (MHI58_12350) recO 2390110..2390880 (-) 771 WP_007501304.1 DNA repair protein RecO Machinery gene
  MHI58_RS12355 (MHI58_12355) - 2390916..2391059 (-) 144 WP_008342408.1 YqzL family protein -
  MHI58_RS12360 (MHI58_12360) era 2391207..2392112 (-) 906 WP_007501305.1 GTPase Era -
  MHI58_RS12365 (MHI58_12365) - 2392105..2392503 (-) 399 WP_008342410.1 cytidine deaminase -
  MHI58_RS12370 (MHI58_12370) - 2392616..2393002 (-) 387 WP_024718818.1 diacylglycerol kinase family protein -
  MHI58_RS12375 (MHI58_12375) ybeY 2392980..2393459 (-) 480 WP_007501310.1 rRNA maturation RNase YbeY -
  MHI58_RS12380 (MHI58_12380) - 2393460..2395550 (-) 2091 WP_173408463.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29345.04 Da        Isoelectric Point: 8.4348

>NTDB_id=986073 MHI58_RS12350 WP_007501304.1 2390110..2390880(-) (recO) [Bacillus sp. FSL K6-2869]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMMESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGLKPELDQCVHCGQKEG
QFHFSIRDNGFICRQCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMDGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=986073 MHI58_RS12350 WP_007501304.1 2390110..2390880(-) (recO) [Bacillus sp. FSL K6-2869]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGGGGCGCTAAGAAATCGAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGTGAAATGATGGAAAGCATGCGAACC
ATTCGGGAAGATTTATTTCTGACCGCATATGCCGCATATATGACAGAATTACTTGATAAAGGAACAGAAGAAAAGAAGCC
AAACCCTTACTTGTTTGAACTTCTTCTTCAATCTTTACGCCATCTCAATGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTTAAAATGCTGTCAGTGATGGGGTTGAAGCCTGAACTGGACCAATGTGTACACTGCGGTCAAAAAGAAGGG
CAGTTCCATTTCTCAATTAGAGATAACGGATTCATCTGTAGGCAATGCTTTTCTAAAGATCCATATAAACTGCCCTTATC
CCCAGCAGCAGCAAGACTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGACGTGAAACCACAAA
CGAAACAAGAAATTCGTCAAGTGCTCGATCATTATTATGAAGAGTATTCAGGTCTTTATTTAAAATCAAAAAAATTCATG
AATCAAATGGAATCCATGAAAAAGCTAATGGACGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.781

100

0.758

Multiple sequence alignment