Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MHB83_RS10205 Genome accession   NZ_CP152034
Coordinates   2035435..2039133 (+) Length   1232 a.a.
NCBI ID   WP_106029932.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3149     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2030435..2044133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB83_RS10190 (MHB83_10190) - 2030728..2031138 (+) 411 WP_106029930.1 DoxX family protein -
  MHB83_RS10195 (MHB83_10195) - 2031336..2031782 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  MHB83_RS10200 (MHB83_10200) addB 2031948..2035457 (+) 3510 WP_342496291.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHB83_RS10205 (MHB83_10205) addA 2035435..2039133 (+) 3699 WP_106029932.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHB83_RS10210 (MHB83_10210) sbcD 2039160..2040335 (+) 1176 WP_044139868.1 exonuclease subunit SbcD -
  MHB83_RS10215 (MHB83_10215) - 2040332..2043727 (+) 3396 WP_342496292.1 SMC family ATPase -
  MHB83_RS10220 (MHB83_10220) - 2043747..2044046 (+) 300 WP_268458930.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 142172.20 Da        Isoelectric Point: 5.0939

>NTDB_id=986004 MHB83_RS10205 WP_106029932.1 2035435..2039133(+) (addA) [Bacillus sp. FSL K6-3149]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKSTDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQPYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDIVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALIDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAEPILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=986004 MHB83_RS10205 WP_106029932.1 2035435..2039133(+) (addA) [Bacillus sp. FSL K6-3149]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCTG
ACCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAATTGGTTGATCGTTATACATCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCGAGATCTCATCCTTCTCCGGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAGCTTTATGAGCTTTTGCCGAACGTTAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTCTCTTAGAAAAATCCACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAATACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAGGGGAA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCAATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGT
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTGGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAATTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCTTGGGCTCCACAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCATTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTATTGTTCATTTAGACGAAAATGAATTGGCTCTGATTCGAACGAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGATACGCAGTACCTTGATTATGTTGGCGGAATGCCTGGAGGTAAGCAGCGACAGGCAAATCTTCGTGCCCTTTATGA
CCGGGCTAAGCAATACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCAGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGGCTCGGCAGAAACTTTAATATGATGGACTTAAACCAGCCCTATTTACTTGA
TAAAGAACTAGGCTTTGGCAGCAAGTACATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAATTAAGGGTCCTATATGTAGCGCTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGAGATATTGTTCTTTCGCATCCGTCTACATTTACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAAAAGGAGCAAGACGAGATTGTACAAGCATTGATCGATGGTCTACCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCAGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAGGACACCTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAGATCCAATCAATTAATCAAGAAGGAATTGCTTCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTAAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGGATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCCTTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGATGGAAA
TTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.289

100

0.666

Multiple sequence alignment