Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHI60_RS06785 Genome accession   NZ_CP152032
Coordinates   1341350..1342729 (-) Length   459 a.a.
NCBI ID   WP_025092231.1    Uniprot ID   A0AAP7QRW5
Organism   Bacillus sp. FSL K6-3200     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1336350..1347729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI60_RS06765 (MHI60_06765) ispF 1337831..1338307 (-) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MHI60_RS06770 (MHI60_06770) ispD 1338311..1339000 (-) 690 WP_003217158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHI60_RS06775 (MHI60_06775) - 1339014..1340114 (-) 1101 WP_095407564.1 PIN/TRAM domain-containing protein -
  MHI60_RS06780 (MHI60_06780) disA 1340268..1341347 (-) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  MHI60_RS06785 (MHI60_06785) radA 1341350..1342729 (-) 1380 WP_025092231.1 DNA repair protein RadA Machinery gene
  MHI60_RS06790 (MHI60_06790) clpC 1342823..1345258 (-) 2436 WP_048240170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MHI60_RS06795 (MHI60_06795) - 1345255..1346346 (-) 1092 WP_144473080.1 protein arginine kinase -
  MHI60_RS06800 (MHI60_06800) - 1346351..1346908 (-) 558 WP_095407549.1 UvrB/UvrC motif-containing protein -
  MHI60_RS06805 (MHI60_06805) - 1346923..1347387 (-) 465 WP_003217184.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50009.49 Da        Isoelectric Point: 7.3829

>NTDB_id=985876 MHI60_RS06785 WP_025092231.1 1341350..1342729(-) (radA) [Bacillus sp. FSL K6-3200]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=985876 MHI60_RS06785 WP_025092231.1 1341350..1342729(-) (radA) [Bacillus sp. FSL K6-3200]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGCCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTGTTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGGAAGTCCACATT
ATTATTACAAGTATCCGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCTTTTGTTGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATAACGTCAGC
TCCCGGTTCTGTATCTCAAGTAAGAGAATGTACAGCACAGCTGATGAAAATTGCCAAGACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCTTCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTCGAGATACAGGCGTTAATTTCCCCGACAAGCTTT
GGGAATCCGCGCAGAATGGCCACTGGCCTTGATCATAATCGAGTGTCGCTCCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCCA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCGAAACTAGGATTCAAGCGAATGTTTATTCC
TCAAGCGAATATAGACGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment