Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI60_RS05440 Genome accession   NZ_CP152032
Coordinates   1094475..1094903 (+) Length   142 a.a.
NCBI ID   WP_144472865.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3200     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1089475..1099903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI60_RS05430 (MHI60_05430) - 1090060..1092048 (-) 1989 WP_144485904.1 hydantoinase B/oxoprolinase family protein -
  MHI60_RS05435 (MHI60_05435) - 1092038..1094107 (-) 2070 WP_144485906.1 hydantoinase/oxoprolinase family protein -
  MHI60_RS05440 (MHI60_05440) nucA/comI 1094475..1094903 (+) 429 WP_144472865.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI60_RS05445 (MHI60_05445) - 1095031..1096728 (+) 1698 WP_144485909.1 methyl-accepting chemotaxis protein -
  MHI60_RS05450 (MHI60_05450) - 1096849..1097235 (+) 387 WP_144485911.1 competence protein ComJ -
  MHI60_RS05455 (MHI60_05455) - 1097237..1098043 (-) 807 WP_144472859.1 AAC(3) family N-acetyltransferase -
  MHI60_RS05460 (MHI60_05460) - 1098073..1098489 (-) 417 WP_048238129.1 Lrp/AsnC family transcriptional regulator -
  MHI60_RS05465 (MHI60_05465) - 1098605..1099369 (+) 765 WP_144485913.1 MBL fold metallo-hydrolase -
  MHI60_RS05470 (MHI60_05470) - 1099414..1099743 (-) 330 WP_095407716.1 YckD family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15856.81 Da        Isoelectric Point: 5.9135

>NTDB_id=985875 MHI60_RS05440 WP_144472865.1 1094475..1094903(+) (nucA/comI) [Bacillus sp. FSL K6-3200]
MKLLKIILLLLLIVIGVATGYIKLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDQS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVSPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=985875 MHI60_RS05440 WP_144472865.1 1094475..1094903(+) (nucA/comI) [Bacillus sp. FSL K6-3200]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTATTAATTGTCATTGGTGTAGCAACAGGCTATATCAAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACCATTCACTTTCCATCAGATCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGCACAATTGACCGTAAACATAGCGATGAGCAGAGAGATCAATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCAATGGCGATGTGTAAAGAAGGGGGCACAGGAGC
TTCTGTCAAATATGTAAGCCCTTCAGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAACTATCAGATGATCCTGATG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.698

74.648

0.535

Multiple sequence alignment