Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHB66_RS11645 Genome accession   NZ_CP152027
Coordinates   2261805..2262875 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0167     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2256805..2267875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB66_RS11595 (MHB66_11595) - 2256899..2257471 (-) 573 WP_034280218.1 signal peptidase I -
  MHB66_RS11600 (MHB66_11600) tapA 2257524..2258051 (-) 528 WP_046311747.1 amyloid fiber anchoring/assembly protein TapA -
  MHB66_RS11605 (MHB66_11605) - 2258351..2258629 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  MHB66_RS11610 (MHB66_11610) - 2258664..2258858 (-) 195 WP_046311750.1 YqzE family protein -
  MHB66_RS11615 (MHB66_11615) comGG 2258918..2259301 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  MHB66_RS11620 (MHB66_11620) comGF 2259298..2259825 (-) 528 WP_259407219.1 competence type IV pilus minor pilin ComGF -
  MHB66_RS11625 (MHB66_11625) comGE 2259737..2260051 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  MHB66_RS11630 (MHB66_11630) comGD 2260035..2260481 (-) 447 WP_046311754.1 competence type IV pilus minor pilin ComGD -
  MHB66_RS11635 (MHB66_11635) comGC 2260474..2260767 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHB66_RS11640 (MHB66_11640) comGB 2260784..2261824 (-) 1041 WP_046311756.1 competence type IV pilus assembly protein ComGB -
  MHB66_RS11645 (MHB66_11645) comGA 2261805..2262875 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  MHB66_RS11650 (MHB66_11650) - 2263053..2263928 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHB66_RS11660 (MHB66_11660) - 2264152..2264532 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHB66_RS11665 (MHB66_11665) - 2264745..2264987 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHB66_RS11670 (MHB66_11670) - 2265033..2265671 (-) 639 WP_046311761.1 MBL fold metallo-hydrolase -
  MHB66_RS11675 (MHB66_11675) - 2265829..2266002 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHB66_RS11680 (MHB66_11680) - 2266226..2266540 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  MHB66_RS11685 (MHB66_11685) - 2266617..2267870 (-) 1254 WP_046311764.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=985639 MHB66_RS11645 WP_041117979.1 2261805..2262875(-) (comGA) [Bacillus sp. FSL L8-0167]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=985639 MHB66_RS11645 WP_041117979.1 2261805..2262875(-) (comGA) [Bacillus sp. FSL L8-0167]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTGATTCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTTGAACAAACAATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAGCTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment