Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHH65_RS11295 Genome accession   NZ_CP152026
Coordinates   2213381..2214451 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0654     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2208381..2219451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH65_RS11245 (MHH65_11245) - 2208475..2209047 (-) 573 WP_034280218.1 signal peptidase I -
  MHH65_RS11250 (MHH65_11250) tapA 2209100..2209627 (-) 528 WP_041117967.1 amyloid fiber anchoring/assembly protein TapA -
  MHH65_RS11255 (MHH65_11255) - 2209927..2210205 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  MHH65_RS11260 (MHH65_11260) - 2210240..2210434 (-) 195 WP_024422974.1 YqzE family protein -
  MHH65_RS11265 (MHH65_11265) comGG 2210494..2210877 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  MHH65_RS11270 (MHH65_11270) comGF 2210874..2211401 (-) 528 WP_264315971.1 competence type IV pilus minor pilin ComGF -
  MHH65_RS11275 (MHH65_11275) comGE 2211313..2211627 (-) 315 WP_264315972.1 competence type IV pilus minor pilin ComGE -
  MHH65_RS11280 (MHH65_11280) comGD 2211611..2212057 (-) 447 WP_034621605.1 competence type IV pilus minor pilin ComGD -
  MHH65_RS11285 (MHH65_11285) comGC 2212050..2212343 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHH65_RS11290 (MHH65_11290) comGB 2212360..2213400 (-) 1041 WP_024422978.1 competence type IV pilus assembly protein ComGB -
  MHH65_RS11295 (MHH65_11295) comGA 2213381..2214451 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  MHH65_RS11300 (MHH65_11300) - 2214632..2215507 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHH65_RS11310 (MHH65_11310) - 2215731..2216111 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHH65_RS11315 (MHH65_11315) - 2216324..2216566 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHH65_RS11320 (MHH65_11320) - 2216612..2217250 (-) 639 WP_034280195.1 MBL fold metallo-hydrolase -
  MHH65_RS11325 (MHH65_11325) - 2217408..2217581 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHH65_RS11330 (MHH65_11330) - 2217806..2218120 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  MHH65_RS11335 (MHH65_11335) - 2218197..2219450 (-) 1254 WP_056767338.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=985578 MHH65_RS11295 WP_041117979.1 2213381..2214451(-) (comGA) [Bacillus sp. FSL L8-0654]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=985578 MHH65_RS11295 WP_041117979.1 2213381..2214451(-) (comGA) [Bacillus sp. FSL L8-0654]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTCTCATTTCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGTTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATTAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATTACGTATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCGCTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment