Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHH65_RS08680 Genome accession   NZ_CP152026
Coordinates   1721738..1722118 (+) Length   126 a.a.
NCBI ID   WP_051149980.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0654     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1716738..1727118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH65_RS08670 (MHH65_08670) - 1717236..1720379 (+) 3144 WP_268414422.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  MHH65_RS08675 (MHH65_08675) - 1720573..1721481 (+) 909 WP_024425124.1 trypsin-like serine protease -
  MHH65_RS08680 (MHH65_08680) nucA/comI 1721738..1722118 (+) 381 WP_051149980.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHH65_RS08685 (MHH65_08685) - 1722263..1723111 (+) 849 WP_024427077.1 STAS domain-containing protein -
  MHH65_RS08690 (MHH65_08690) - 1723144..1723686 (-) 543 WP_024425127.1 IseA DL-endopeptidase inhibitor family protein -
  MHH65_RS08695 (MHH65_08695) - 1723983..1724432 (+) 450 WP_024427079.1 hypothetical protein -
  MHH65_RS08700 (MHH65_08700) lexA 1724477..1725097 (-) 621 WP_024427080.1 transcriptional repressor LexA -
  MHH65_RS08705 (MHH65_08705) yneA 1725256..1725567 (+) 312 WP_024425130.1 cell division suppressor protein YneA -
  MHH65_RS08710 (MHH65_08710) - 1725585..1726232 (+) 648 WP_025093247.1 recombinase family protein -
  MHH65_RS08715 (MHH65_08715) - 1726302..1726532 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13761.43 Da        Isoelectric Point: 8.5068

>NTDB_id=985572 MHH65_RS08680 WP_051149980.1 1721738..1722118(+) (nucA/comI) [Bacillus sp. FSL L8-0654]
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=985572 MHH65_RS08680 WP_051149980.1 1721738..1722118(+) (nucA/comI) [Bacillus sp. FSL L8-0654]
ATGGGCACGTTGTTTGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCGGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCATCAAATCAGCGGATTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment