Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHH65_RS01945 Genome accession   NZ_CP152026
Coordinates   366518..366946 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. FSL L8-0654     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 361518..371946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH65_RS01915 (MHH65_01915) - 361681..362010 (+) 330 WP_087978414.1 YckD family protein -
  MHH65_RS01920 (MHH65_01920) - 362054..362818 (-) 765 WP_098677316.1 MBL fold metallo-hydrolase -
  MHH65_RS01925 (MHH65_01925) - 362934..363350 (+) 417 WP_176544459.1 Lrp/AsnC family transcriptional regulator -
  MHH65_RS01930 (MHH65_01930) - 363379..364185 (+) 807 WP_025092812.1 AAC(3) family N-acetyltransferase -
  MHH65_RS01935 (MHH65_01935) - 364187..364573 (-) 387 WP_056765076.1 competence protein ComJ -
  MHH65_RS01940 (MHH65_01940) - 364693..366390 (-) 1698 WP_268414325.1 methyl-accepting chemotaxis protein -
  MHH65_RS01945 (MHH65_01945) nucA/comI 366518..366946 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHH65_RS01950 (MHH65_01950) - 367314..369383 (+) 2070 WP_342497886.1 hydantoinase/oxoprolinase family protein -
  MHH65_RS01955 (MHH65_01955) - 369373..371361 (+) 1989 WP_098677312.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=985546 MHH65_RS01945 WP_024424305.1 366518..366946(-) (nucA/comI) [Bacillus sp. FSL L8-0654]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=985546 MHH65_RS01945 WP_024424305.1 366518..366946(-) (nucA/comI) [Bacillus sp. FSL L8-0654]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAAAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment