Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACLB14_RS02575 Genome accession   NZ_CP184155
Coordinates   533544..534977 (-) Length   477 a.a.
NCBI ID   WP_233324198.1    Uniprot ID   -
Organism   Escherichia coli strain C21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528544..539977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB14_RS02540 (ACLB14_02540) elbB 529235..529888 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACLB14_RS02545 (ACLB14_02545) mtgA 529885..530613 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACLB14_RS02550 (ACLB14_02550) yrbL 530610..531242 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACLB14_RS02555 (ACLB14_02555) npr 531456..531728 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACLB14_RS02560 (ACLB14_02560) rapZ 531725..532579 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACLB14_RS02565 (ACLB14_02565) ptsN 532625..533116 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACLB14_RS02570 (ACLB14_02570) hpf 533234..533521 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACLB14_RS02575 (ACLB14_02575) rpoN/rpoN1 533544..534977 (-) 1434 WP_233324198.1 RNA polymerase factor sigma-54 Machinery gene
  ACLB14_RS02580 (ACLB14_02580) lptB 535025..535750 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACLB14_RS02585 (ACLB14_02585) lptA 535757..536314 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACLB14_RS02590 (ACLB14_02590) lptC 536283..536858 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACLB14_RS02595 (ACLB14_02595) kdsC 536855..537421 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACLB14_RS02600 (ACLB14_02600) kdsD 537442..538428 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACLB14_RS02605 (ACLB14_02605) yrbG 538442..539419 (-) 978 WP_233324200.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54047.82 Da        Isoelectric Point: 4.3473

>NTDB_id=985546 ACLB14_RS02575 WP_233324198.1 533544..534977(-) (rpoN/rpoN1) [Escherichia coli strain C21]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSDTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=985546 ACLB14_RS02575 WP_233324198.1 533544..534977(-) (rpoN/rpoN1) [Escherichia coli strain C21]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTATTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGACACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.93

100

0.474

Multiple sequence alignment