Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHH65_RS00590 Genome accession   NZ_CP152026
Coordinates   109197..110576 (+) Length   459 a.a.
NCBI ID   WP_024425863.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0654     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104197..115576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH65_RS00570 (MHH65_00570) - 104539..105003 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  MHH65_RS00575 (MHH65_00575) - 105018..105575 (+) 558 WP_024425862.1 UvrB/UvrC motif-containing protein -
  MHH65_RS00580 (MHH65_00580) - 105580..106671 (+) 1092 WP_024425579.1 protein arginine kinase -
  MHH65_RS00585 (MHH65_00585) clpC 106668..109103 (+) 2436 WP_048240170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MHH65_RS00590 (MHH65_00590) radA 109197..110576 (+) 1380 WP_024425863.1 DNA repair protein RadA Machinery gene
  MHH65_RS00595 (MHH65_00595) disA 110579..111658 (+) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  MHH65_RS00600 (MHH65_00600) - 111812..112912 (+) 1101 WP_024425582.1 PIN/TRAM domain-containing protein -
  MHH65_RS00605 (MHH65_00605) ispD 112926..113615 (+) 690 WP_024425583.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHH65_RS00610 (MHH65_00610) ispF 113619..114095 (+) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50009.49 Da        Isoelectric Point: 7.3829

>NTDB_id=985545 MHH65_RS00590 WP_024425863.1 109197..110576(+) (radA) [Bacillus sp. FSL L8-0654]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANLDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=985545 MHH65_RS00590 WP_024425863.1 109197..110576(+) (radA) [Bacillus sp. FSL L8-0654]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACAAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCTCTTGTTCTCATCGGCGGAGATCCTGGGATTGGGAAGTCCACATT
GTTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCTATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCGTTTGTTGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
TCCCGGCTCTGTATCTCAAGTTAGAGAATGTACAGCACAGCTGATGAAGATTGCCAAAACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATTTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTCGAGATACAGGCATTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGATCATAATCGAGTATCGCTGCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCCTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCTA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCAAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATCTAGACGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.555

99.782

0.874
  radA Streptococcus mitis NCTC 12261

61.81

98.693

0.61
  radA Streptococcus pneumoniae Rx1

61.81

98.693

0.61
  radA Streptococcus pneumoniae D39

61.81

98.693

0.61
  radA Streptococcus pneumoniae R6

61.81

98.693

0.61
  radA Streptococcus pneumoniae TIGR4

61.81

98.693

0.61
  radA Streptococcus mitis SK321

61.81

98.693

0.61

Multiple sequence alignment