Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLB14_RS01915 Genome accession   NZ_CP184155
Coordinates   413445..414122 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain C21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408445..419122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB14_RS01900 (ACLB14_01900) chiA 409812..412505 (+) 2694 WP_020239449.1 bifunctional chitinase/lysozyme -
  ACLB14_RS01905 (ACLB14_01905) bfd 412674..412868 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  ACLB14_RS01910 (ACLB14_01910) bfr 412940..413416 (+) 477 WP_000675504.1 bacterioferritin -
  ACLB14_RS01915 (ACLB14_01915) pilD 413445..414122 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  ACLB14_RS01920 (ACLB14_01920) gspM 414122..414583 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  ACLB14_RS01925 (ACLB14_01925) gspL 414580..415743 (-) 1164 WP_001618895.1 type II secretion system protein GspL -
  ACLB14_RS01930 (ACLB14_01930) gspK 415758..416741 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  ACLB14_RS01935 (ACLB14_01935) gspJ 416734..417321 (-) 588 WP_233324246.1 type II secretion system minor pseudopilin GspJ -
  ACLB14_RS01940 (ACLB14_01940) gspI 417314..417691 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  ACLB14_RS01945 (ACLB14_01945) gspH 417688..418197 (-) 510 WP_001618891.1 type II secretion system minor pseudopilin GspH -
  ACLB14_RS01950 (ACLB14_01950) gspG 418205..418642 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=985543 ACLB14_RS01915 WP_000178161.1 413445..414122(-) (pilD) [Escherichia coli strain C21]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=985543 ACLB14_RS01915 WP_000178161.1 413445..414122(-) (pilD) [Escherichia coli strain C21]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment