Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI23_RS11530 Genome accession   NZ_CP152024
Coordinates   2256302..2257372 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2251302..2262372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI23_RS11480 (MHI23_11480) - 2251396..2251968 (-) 573 WP_024422971.1 signal peptidase I -
  MHI23_RS11485 (MHI23_11485) tapA 2252021..2252548 (-) 528 WP_326161475.1 amyloid fiber anchoring/assembly protein TapA -
  MHI23_RS11490 (MHI23_11490) - 2252848..2253126 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  MHI23_RS11495 (MHI23_11495) - 2253161..2253355 (-) 195 WP_056767455.1 YqzE family protein -
  MHI23_RS11500 (MHI23_11500) comGG 2253415..2253798 (-) 384 WP_125586574.1 competence type IV pilus minor pilin ComGG -
  MHI23_RS11505 (MHI23_11505) comGF 2253795..2254322 (-) 528 WP_335374388.1 competence type IV pilus minor pilin ComGF -
  MHI23_RS11510 (MHI23_11510) comGE 2254234..2254548 (-) 315 WP_025092974.1 competence type IV pilus minor pilin ComGE -
  MHI23_RS11515 (MHI23_11515) comGD 2254532..2254978 (-) 447 WP_125586578.1 competence type IV pilus minor pilin ComGD -
  MHI23_RS11520 (MHI23_11520) comGC 2254971..2255264 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI23_RS11525 (MHI23_11525) comGB 2255281..2256321 (-) 1041 WP_125586580.1 competence type IV pilus assembly protein ComGB -
  MHI23_RS11530 (MHI23_11530) comGA 2256302..2257372 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI23_RS11535 (MHI23_11535) - 2257550..2258425 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHI23_RS11545 (MHI23_11545) - 2258649..2259029 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI23_RS11550 (MHI23_11550) - 2259243..2259485 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI23_RS11555 (MHI23_11555) - 2259533..2260171 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  MHI23_RS11560 (MHI23_11560) - 2260329..2260502 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHI23_RS11565 (MHI23_11565) - 2260528..2260842 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  MHI23_RS11570 (MHI23_11570) - 2260921..2262174 (-) 1254 WP_342491969.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=985455 MHI23_RS11530 WP_041117979.1 2256302..2257372(-) (comGA) [Bacillus sp. FSL M7-0003]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=985455 MHI23_RS11530 WP_041117979.1 2256302..2257372(-) (comGA) [Bacillus sp. FSL M7-0003]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGACTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGTTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCTGGATTACGCGCTATATTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCGCTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTATCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment