Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI23_RS01965 Genome accession   NZ_CP152024
Coordinates   370818..371246 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. FSL M7-0003     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365818..376246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI23_RS01935 (MHI23_01935) - 365981..366310 (+) 330 WP_024424311.1 YckD family protein -
  MHI23_RS01940 (MHI23_01940) - 366354..367118 (-) 765 WP_149126012.1 MBL fold metallo-hydrolase -
  MHI23_RS01945 (MHI23_01945) - 367234..367650 (+) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  MHI23_RS01950 (MHI23_01950) - 367679..368485 (+) 807 WP_149126011.1 AAC(3) family N-acetyltransferase -
  MHI23_RS01955 (MHI23_01955) - 368487..368873 (-) 387 WP_149126010.1 competence protein ComJ -
  MHI23_RS01960 (MHI23_01960) - 368993..370690 (-) 1698 WP_056765079.1 methyl-accepting chemotaxis protein -
  MHI23_RS01965 (MHI23_01965) nucA/comI 370818..371246 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI23_RS01970 (MHI23_01970) - 371614..373683 (+) 2070 WP_024427271.1 hydantoinase/oxoprolinase family protein -
  MHI23_RS01975 (MHI23_01975) - 373673..375661 (+) 1989 WP_254968264.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=985424 MHI23_RS01965 WP_024424305.1 370818..371246(-) (nucA/comI) [Bacillus sp. FSL M7-0003]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=985424 MHI23_RS01965 WP_024424305.1 370818..371246(-) (nucA/comI) [Bacillus sp. FSL M7-0003]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment