Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHI08_RS00590 Genome accession   NZ_CP152022
Coordinates   109517..110896 (+) Length   459 a.a.
NCBI ID   WP_342499887.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0791     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104517..115896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI08_RS00570 (MHI08_00570) - 104859..105323 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  MHI08_RS00575 (MHI08_00575) - 105338..105895 (+) 558 WP_024425862.1 UvrB/UvrC motif-containing protein -
  MHI08_RS00580 (MHI08_00580) - 105900..106991 (+) 1092 WP_024425579.1 protein arginine kinase -
  MHI08_RS00585 (MHI08_00585) clpC 106988..109423 (+) 2436 WP_007496263.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MHI08_RS00590 (MHI08_00590) radA 109517..110896 (+) 1380 WP_342499887.1 DNA repair protein RadA Machinery gene
  MHI08_RS00595 (MHI08_00595) disA 110899..111978 (+) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  MHI08_RS00600 (MHI08_00600) - 112132..113232 (+) 1101 WP_024425582.1 PIN/TRAM domain-containing protein -
  MHI08_RS00605 (MHI08_00605) ispD 113246..113935 (+) 690 WP_024425583.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHI08_RS00610 (MHI08_00610) ispF 113939..114415 (+) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49995.46 Da        Isoelectric Point: 7.3829

>NTDB_id=985302 MHI08_RS00590 WP_342499887.1 109517..110896(+) (radA) [Bacillus sp. FSL M7-0791]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFVPQANLDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=985302 MHI08_RS00590 WP_342499887.1 109517..110896(+) (radA) [Bacillus sp. FSL M7-0791]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACAAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCTCTTGTTCTCATCGGCGGAGATCCTGGGATTGGGAAGTCCACATT
GTTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCGTTTGTTGTGGTGGATTCGATTCAAACCGTTTACCAAAGCGATATCACGTCAGC
TCCCGGCTCTGTATCTCAAGTTAGAGAATGTACAGCGCAGCTGATGAAGATTGCCAAGACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTAACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGATACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAATCCATCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTCGAGATACAGGCATTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGATCATAATCGAGTATCGCTGCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCCTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCGATTGACTTAGCTA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCAAAACTAGGATTTAAGCGAATGTTTGTTCC
TCAGGCGAATCTAGACGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

62.031

98.693

0.612
  radA Streptococcus pneumoniae Rx1

62.031

98.693

0.612
  radA Streptococcus pneumoniae D39

62.031

98.693

0.612
  radA Streptococcus pneumoniae R6

62.031

98.693

0.612
  radA Streptococcus pneumoniae TIGR4

62.031

98.693

0.612
  radA Streptococcus mitis SK321

62.031

98.693

0.612

Multiple sequence alignment