Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   MKY83_RS08285 Genome accession   NZ_CP152015
Coordinates   1646795..1647697 (+) Length   300 a.a.
NCBI ID   WP_065097271.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0266     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1641795..1652697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY83_RS08265 (MKY83_08265) - 1642461..1644197 (+) 1737 WP_185107244.1 glycosyltransferase -
  MKY83_RS08270 (MKY83_08270) - 1644194..1644469 (+) 276 WP_065097270.1 FlhB-like flagellar biosynthesis protein -
  MKY83_RS08275 (MKY83_08275) sucC 1644646..1645806 (+) 1161 WP_012009945.1 ADP-forming succinate--CoA ligase subunit beta -
  MKY83_RS08280 (MKY83_08280) sucD 1645829..1646731 (+) 903 WP_003212080.1 succinate--CoA ligase subunit alpha -
  MKY83_RS08285 (MKY83_08285) dprA 1646795..1647697 (+) 903 WP_065097271.1 DNA-processing protein DprA Machinery gene
  MKY83_RS08290 (MKY83_08290) topA 1647891..1649966 (+) 2076 WP_044141928.1 type I DNA topoisomerase -
  MKY83_RS08295 (MKY83_08295) trmFO 1650037..1651341 (+) 1305 WP_003212444.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  MKY83_RS08300 (MKY83_08300) xerC 1651404..1652321 (+) 918 WP_106067464.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34133.60 Da        Isoelectric Point: 9.1124

>NTDB_id=985025 MKY83_RS08285 WP_065097271.1 1646795..1647697(+) (dprA) [Bacillus sp. FSL M8-0266]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPKLYINEETHHFKQDRSLQTIDFTRLKQAEEKEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKHIYDPPPVLFLKGNVSYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEDWMIVSGLAKGIDGLAH
KECIRNKGKTIGIIAGGFQHLYPKEHVQMAEYMGEHHLLLSEHPPYVKPEKWHFPLRNRLISALTSGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDPNSTGPARLIQQGAKLVHSTKDILEEFSFRSVQYTELL

Nucleotide


Download         Length: 903 bp        

>NTDB_id=985025 MKY83_RS08285 WP_065097271.1 1646795..1647697(+) (dprA) [Bacillus sp. FSL M8-0266]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCTTTGTTAACAAAATGGTGGAA
AGTCGATCCTAAGCTATATATAAATGAAGAAACACATCATTTCAAACAGGATCGATCATTACAAACAATCGACTTTACCC
GCTTAAAACAAGCCGAAGAAAAGGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCTTATATCCCAGCACACTAAAACATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CGTGTCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTAGGCACGCGTGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAACTTGTTAAGGAAGATTGGATGATTGTCAGTGGCTTAGCAAAAGGCATTGATGGACTTGCACAT
AAAGAGTGCATTAGGAATAAAGGGAAAACGATCGGTATTATAGCAGGCGGATTTCAGCATTTATACCCAAAGGAACATGT
GCAAATGGCTGAATACATGGGCGAGCATCATTTGCTTTTATCCGAGCATCCGCCTTATGTCAAACCAGAAAAGTGGCATT
TCCCTTTGAGGAATCGTTTAATTAGTGCGCTGACAAGTGGGACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCACTC
ATTACCGCGTATCAAGCACTTGAGCAAGGCAAAGAGGTATTTGCGGTTGCAGGATCAATCTTTGATCCTAATTCCACAGG
TCCAGCCAGACTTATACAGCAGGGAGCAAAGCTTGTTCATTCAACGAAGGATATTTTAGAGGAATTCTCCTTCCGCAGTG
TTCAATATACTGAACTCTTATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.704

95.667

0.523
  dprA Lactococcus lactis subsp. cremoris KW2

38.542

96

0.37

Multiple sequence alignment