Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MKX52_RS11380 Genome accession   NZ_CP152014
Coordinates   2227983..2228753 (-) Length   256 a.a.
NCBI ID   WP_012010612.1    Uniprot ID   A8FFB2
Organism   Bacillus sp. FSL R5-0422     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2222983..2233753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX52_RS11360 (MKX52_11360) - 2223076..2223897 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  MKX52_RS11365 (MKX52_11365) - 2223923..2224561 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  MKX52_RS11370 (MKX52_11370) glyS 2224728..2226773 (-) 2046 WP_342490721.1 glycine--tRNA ligase subunit beta -
  MKX52_RS11375 (MKX52_11375) glyQ 2226766..2227653 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  MKX52_RS11380 (MKX52_11380) recO 2227983..2228753 (-) 771 WP_012010612.1 DNA repair protein RecO Machinery gene
  MKX52_RS11385 (MKX52_11385) - 2228789..2228932 (-) 144 WP_003217288.1 YqzL family protein -
  MKX52_RS11390 (MKX52_11390) era 2229081..2229986 (-) 906 WP_012010613.1 GTPase Era -
  MKX52_RS11395 (MKX52_11395) - 2229979..2230377 (-) 399 WP_342490722.1 cytidine deaminase -
  MKX52_RS11400 (MKX52_11400) - 2230491..2230877 (-) 387 WP_041815743.1 diacylglycerol kinase family protein -
  MKX52_RS11405 (MKX52_11405) ybeY 2230855..2231334 (-) 480 WP_342490723.1 rRNA maturation RNase YbeY -
  MKX52_RS11410 (MKX52_11410) - 2231335..2233422 (-) 2088 WP_049754949.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29230.90 Da        Isoelectric Point: 8.4348

>NTDB_id=984975 MKX52_RS11380 WP_012010612.1 2227983..2228753(-) (recO) [Bacillus sp. FSL R5-0422]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=984975 MKX52_RS11380 WP_012010612.1 2227983..2228753(-) (recO) [Bacillus sp. FSL R5-0422]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGTGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGATTTATTTTTGACCGCATATGCCGCATACATGACAGAATTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCTTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTTAAAATGTTATCAGTGATGGGGATGAAGCCCGAACTTGATCAATGTGTTCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCAATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAAA
CGAAACAAGAAATTCGTCAAGTGCTCGACCACTACTATGACGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAAATGGAATCCATGAAAAAGCTCATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FFB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment