Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKX52_RS11105 Genome accession   NZ_CP152014
Coordinates   2177537..2178607 (-) Length   356 a.a.
NCBI ID   WP_088000364.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0422     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2172537..2183607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX52_RS11055 (MKX52_11055) - 2172630..2173202 (-) 573 WP_342490700.1 signal peptidase I -
  MKX52_RS11060 (MKX52_11060) tapA 2173254..2173781 (-) 528 WP_342490701.1 amyloid fiber anchoring/assembly protein TapA -
  MKX52_RS11065 (MKX52_11065) - 2174081..2174359 (+) 279 WP_061827416.1 DUF3889 domain-containing protein -
  MKX52_RS11070 (MKX52_11070) - 2174393..2174587 (-) 195 WP_012010560.1 YqzE family protein -
  MKX52_RS11075 (MKX52_11075) comGG 2174647..2175030 (-) 384 WP_342490702.1 competence type IV pilus minor pilin ComGG -
  MKX52_RS11080 (MKX52_11080) comGF 2175027..2175527 (-) 501 WP_342490703.1 competence type IV pilus minor pilin ComGF -
  MKX52_RS11085 (MKX52_11085) comGE 2175466..2175783 (-) 318 WP_012010563.1 competence type IV pilus minor pilin ComGE -
  MKX52_RS11090 (MKX52_11090) comGD 2175767..2176213 (-) 447 WP_342490704.1 competence type IV pilus minor pilin ComGD -
  MKX52_RS11095 (MKX52_11095) comGC 2176206..2176499 (-) 294 WP_012010565.1 competence type IV pilus major pilin ComGC Machinery gene
  MKX52_RS11100 (MKX52_11100) comGB 2176516..2177595 (-) 1080 WP_342490705.1 competence type IV pilus assembly protein ComGB -
  MKX52_RS11105 (MKX52_11105) comGA 2177537..2178607 (-) 1071 WP_088000364.1 competence type IV pilus ATPase ComGA Machinery gene
  MKX52_RS11110 (MKX52_11110) - 2178785..2179657 (-) 873 WP_342490706.1 STAS domain-containing protein -
  MKX52_RS11120 (MKX52_11120) - 2179880..2180260 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKX52_RS11125 (MKX52_11125) - 2180474..2180716 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MKX52_RS11130 (MKX52_11130) - 2180959..2181597 (-) 639 WP_251184271.1 MBL fold metallo-hydrolase -
  MKX52_RS11135 (MKX52_11135) - 2181755..2181928 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MKX52_RS11140 (MKX52_11140) - 2182153..2182467 (-) 315 WP_088000368.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40255.59 Da        Isoelectric Point: 9.0692

>NTDB_id=984974 MKX52_RS11105 WP_088000364.1 2177537..2178607(-) (comGA) [Bacillus sp. FSL R5-0422]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFVSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIDLTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=984974 MKX52_RS11105 WP_088000364.1 2177537..2178607(-) (comGA) [Bacillus sp. FSL R5-0422]
TTGTATGGGATTGAATATTTAGGACAAGAGCTGCTAGAAGAAGCATGCCGAATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGACTTAATTCAGCAAAGAACTATTGATAAAAAAAGCGGCG
AGCGACTCATTGCTCATTTTAAATTTGTATCTTCTATGGATATTGGGGAAAAAAGGAGACCTCAAAATGGATCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCACAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCACCAGATTATTATCGTTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTCTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAATCGAAATATTATTACACTTGAAGATCCAGTAGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGGCATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCCGAACGGCAATTAGAGCAGCGCTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGTATGCTTGAATTTGGTGTCACAATGAATGAACTTGAACAAACAATGGTGGC
GATTGCTGCGCAGCGATTAATTGATCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATTAAGGAGGCAAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment