Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKX52_RS08465 Genome accession   NZ_CP152014
Coordinates   1692639..1693019 (+) Length   126 a.a.
NCBI ID   WP_224925450.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0422     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1687639..1698019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX52_RS08455 (MKX52_08455) - 1688130..1691273 (+) 3144 WP_342491793.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  MKX52_RS08460 (MKX52_08460) - 1691468..1692376 (+) 909 WP_342491794.1 trypsin-like serine protease -
  MKX52_RS08465 (MKX52_08465) nucA/comI 1692639..1693019 (+) 381 WP_224925450.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MKX52_RS08470 (MKX52_08470) - 1693158..1694006 (+) 849 WP_012010093.1 STAS domain-containing protein -
  MKX52_RS08475 (MKX52_08475) - 1694039..1694581 (-) 543 WP_144468527.1 IseA DL-endopeptidase inhibitor family protein -
  MKX52_RS08480 (MKX52_08480) - 1694876..1695310 (+) 435 WP_144503242.1 hypothetical protein -
  MKX52_RS08485 (MKX52_08485) lexA 1695356..1695976 (-) 621 WP_144503240.1 transcriptional repressor LexA -
  MKX52_RS08490 (MKX52_08490) yneA 1696136..1696447 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  MKX52_RS08495 (MKX52_08495) - 1696465..1697112 (+) 648 WP_012010098.1 recombinase family protein -
  MKX52_RS08500 (MKX52_08500) - 1697182..1697412 (+) 231 WP_003211509.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13799.45 Da        Isoelectric Point: 7.3832

>NTDB_id=984968 MKX52_RS08465 WP_224925450.1 1692639..1693019(+) (nucA/comI) [Bacillus sp. FSL R5-0422]
MGTLLGGFGEEHAAKGAERYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQMSGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=984968 MKX52_RS08465 WP_224925450.1 1692639..1693019(+) (nucA/comI) [Bacillus sp. FSL R5-0422]
ATGGGCACGCTGTTAGGTGGTTTTGGGGAGGAACATGCAGCAAAAGGCGCGGAACGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTATCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCTTTAAAAGGCATTCCGACTAAACCAGGATTCGACCGAGATGAGTGGCCA
ATGGCCGTTTGTCTTGAAGGAGGAAAAGGGGCGAGTGTTCAATATGTCAGCCCGTCTGACAATAGGGGAGCAGGCTCCTG
GGTCGGACACCAGATGAGTGGGTATCCTGATGGGAAAAGAATATTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment