Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKY18_RS08745 Genome accession   NZ_CP152010
Coordinates   1771688..1772068 (+) Length   126 a.a.
NCBI ID   WP_223250648.1    Uniprot ID   -
Organism   Bacillus sp. FSL R7-0672     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1766688..1777068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY18_RS08735 (MKY18_08735) - 1767178..1770321 (+) 3144 WP_113767722.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  MKY18_RS08740 (MKY18_08740) - 1770517..1771425 (+) 909 WP_044141413.1 trypsin-like serine protease -
  MKY18_RS08745 (MKY18_08745) nucA/comI 1771688..1772068 (+) 381 WP_223250648.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MKY18_RS08750 (MKY18_08750) - 1772218..1773066 (+) 849 WP_044141412.1 STAS domain-containing protein -
  MKY18_RS08755 (MKY18_08755) - 1773100..1773642 (-) 543 WP_065097361.1 IseA DL-endopeptidase inhibitor family protein -
  MKY18_RS08760 (MKY18_08760) - 1773937..1774371 (+) 435 WP_185108836.1 hypothetical protein -
  MKY18_RS08765 (MKY18_08765) lexA 1774417..1775037 (-) 621 WP_065097363.1 transcriptional repressor LexA -
  MKY18_RS08770 (MKY18_08770) yneA 1775197..1775508 (+) 312 WP_185108834.1 cell division suppressor protein YneA -
  MKY18_RS08775 (MKY18_08775) - 1775526..1776173 (+) 648 WP_003211322.1 recombinase family protein -
  MKY18_RS08780 (MKY18_08780) - 1776243..1776473 (+) 231 WP_039167715.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13829.48 Da        Isoelectric Point: 7.3831

>NTDB_id=984683 MKY18_RS08745 WP_223250648.1 1771688..1772068(+) (nucA/comI) [Bacillus sp. FSL R7-0672]
MVTLLGGFGEEHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWTGHQMSGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=984683 MKY18_RS08745 WP_223250648.1 1771688..1772068(+) (nucA/comI) [Bacillus sp. FSL R7-0672]
ATGGTCACGCTGTTAGGTGGATTTGGGGAGGAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGATACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCCGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGCATTCCGACTAAACCCGGATTCGACCGAGATGAGTGGCCG
ATGGCAGTTTGTCTTGAAGGAGGAAAAGGGGCGAGTGTTCAATATGTCAGCCCGTCTGATAATAGGGGAGCGGGCTCATG
GACCGGACACCAGATGAGCGGGTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.143

94.444

0.54

Multiple sequence alignment