Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MKZ22_RS12945 Genome accession   NZ_CP152009
Coordinates   2489367..2490137 (-) Length   256 a.a.
NCBI ID   WP_342487376.1    Uniprot ID   -
Organism   Bacillus sp. FSL R7-0675     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2484367..2495137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ22_RS12925 (MKZ22_12925) - 2484460..2485281 (-) 822 WP_044139409.1 pyruvate, water dikinase regulatory protein -
  MKZ22_RS12930 (MKZ22_12930) - 2485307..2485945 (-) 639 WP_034620854.1 helix-turn-helix transcriptional regulator -
  MKZ22_RS12935 (MKZ22_12935) glyS 2486112..2488157 (-) 2046 WP_342487375.1 glycine--tRNA ligase subunit beta -
  MKZ22_RS12940 (MKZ22_12940) glyQ 2488150..2489037 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  MKZ22_RS12945 (MKZ22_12945) recO 2489367..2490137 (-) 771 WP_342487376.1 DNA repair protein RecO Machinery gene
  MKZ22_RS12950 (MKZ22_12950) - 2490172..2490315 (-) 144 WP_065098441.1 YqzL family protein -
  MKZ22_RS12955 (MKZ22_12955) era 2490464..2491369 (-) 906 WP_065098416.1 GTPase Era -
  MKZ22_RS12960 (MKZ22_12960) - 2491362..2491760 (-) 399 WP_342487377.1 cytidine deaminase -
  MKZ22_RS12965 (MKZ22_12965) - 2491874..2492260 (-) 387 WP_044139413.1 diacylglycerol kinase family protein -
  MKZ22_RS12970 (MKZ22_12970) ybeY 2492238..2492717 (-) 480 WP_044139414.1 rRNA maturation RNase YbeY -
  MKZ22_RS12975 (MKZ22_12975) - 2492718..2494805 (-) 2088 WP_050943969.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29274.95 Da        Isoelectric Point: 8.4348

>NTDB_id=984629 MKZ22_RS12945 WP_342487376.1 2489367..2490137(-) (recO) [Bacillus sp. FSL R7-0675]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKSTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=984629 MKZ22_RS12945 WP_342487376.1 2489367..2490137(-) (recO) [Bacillus sp. FSL R7-0675]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTCTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAAGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTTTGCGTCATCTGAATGAAGGTACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGAATGAAGCCCGAACTGGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCGATTAGAGACAACGGCTTTATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
TCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTCATGGGTGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment