Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MKX67_RS00560 Genome accession   NZ_CP151998
Coordinates   108185..109564 (+) Length   459 a.a.
NCBI ID   WP_095371250.1    Uniprot ID   -
Organism   Cytobacillus sp. FSL W7-1323     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 103185..114564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX67_RS00540 (MKX67_00540) - 103455..103916 (+) 462 WP_095371254.1 CtsR family transcriptional regulator -
  MKX67_RS00545 (MKX67_00545) - 103944..104492 (+) 549 WP_095371253.1 UvrB/UvrC motif-containing protein -
  MKX67_RS00550 (MKX67_00550) - 104495..105574 (+) 1080 WP_095371252.1 protein arginine kinase -
  MKX67_RS00555 (MKX67_00555) clpC 105581..108025 (+) 2445 WP_095371251.1 ATP-dependent protease ATP-binding subunit ClpC -
  MKX67_RS00560 (MKX67_00560) radA 108185..109564 (+) 1380 WP_095371250.1 DNA repair protein RadA Machinery gene
  MKX67_RS00565 (MKX67_00565) disA 109569..110642 (+) 1074 WP_095371249.1 DNA integrity scanning diadenylate cyclase DisA -
  MKX67_RS00570 (MKX67_00570) - 110860..111942 (+) 1083 WP_307317049.1 PIN/TRAM domain-containing protein -
  MKX67_RS00575 (MKX67_00575) ispD 112030..112719 (+) 690 WP_340977150.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MKX67_RS00580 (MKX67_00580) ispF 112733..113212 (+) 480 WP_322910605.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50223.78 Da        Isoelectric Point: 7.4605

>NTDB_id=984139 MKX67_RS00560 WP_095371250.1 108185..109564(+) (radA) [Cytobacillus sp. FSL W7-1323]
MAKRKSKFICQECGYESAKWMGKCPGCGVWNKMTEEIEKPANSRRGAFAHTQETTIRAKATPIPKIETVNEPRIYTEMKE
LNRVLGGGVVRGSLVLIGGDPGIGKSTLLLQVSSQLSNKQHSVLYISGEESIRQTKLRADRLNISSEHLLVYAETSLEEI
SQTIDEVKPEFVIIDSIQTIYHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKELGLEEVANPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDKPTRATDCIIGEVGL
TGEIRRVSRIEQRVQEAAKLGFERIILPANNLSGWTGPKNVQLIGVHSVSEALEAALGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=984139 MKX67_RS00560 WP_095371250.1 108185..109564(+) (radA) [Cytobacillus sp. FSL W7-1323]
TTGGCGAAAAGAAAATCAAAATTCATATGTCAAGAATGTGGTTATGAATCGGCAAAGTGGATGGGGAAATGTCCCGGATG
CGGTGTATGGAATAAAATGACGGAAGAGATAGAGAAACCTGCTAACTCGAGAAGAGGGGCTTTTGCACATACACAAGAGA
CAACGATTAGAGCAAAAGCGACTCCGATTCCAAAAATTGAAACAGTCAATGAGCCACGTATTTATACGGAAATGAAAGAG
TTAAATCGAGTCCTTGGTGGTGGGGTTGTTAGAGGATCTCTCGTTTTAATAGGTGGAGATCCTGGGATTGGAAAATCAAC
ACTACTGTTGCAAGTTTCTTCTCAATTATCTAATAAACAGCATTCTGTACTTTATATTTCTGGAGAAGAGTCGATAAGAC
AAACGAAGCTACGAGCTGACAGATTAAATATTTCATCAGAACATCTACTTGTTTATGCAGAGACAAGCTTAGAAGAGATT
AGTCAAACGATTGATGAGGTGAAGCCGGAGTTTGTTATTATTGACTCCATTCAAACTATTTATCACCCGGAAGTGACCTC
GGCTCCTGGTAGTGTTTCACAGGTAAGAGAGTGTACGGCTGAGCTTATGAGAATCGGGAAAACGAAAGGGATTGCGATCT
TTATTGTTGGACATGTAACGAAAGAAGGATCGATCGCCGGTCCTAGGTTGCTTGAACATATGGTGGATACGGTGCTTTAT
TTTGAAGGGGAAAGGCATCACACGTATCGAATTTTAAGAGCAGTCAAAAACCGATTTGGTTCTACGAATGAAATGGGTAT
ATTTGAAATGAAAGAATTAGGTTTAGAAGAAGTGGCAAACCCTTCAGAGATTTTTTTAGAAGAACGATCACAAGGAGCAG
CGGGTTCAACTGTTGTGGCTTCAATGGAAGGAACTCGGCCCGTTCTTGTAGAAATACAAGCATTAATATCCCCTACGAGT
TTTGGTAACCCAAGAAGGATGGCAACAGGGATTGATCATAACCGTGTGCCGCTATTAATGGCGGTATTAGAGAAACGGGT
CGGTATGTTGTTACAAAATCAAGATGCTTATTTGAAGGTTGCTGGAGGGGTAAAACTAGATGAGCCAGCAATTGATTTGG
CAGTTGCAGTTAGTATCGCATCAAGCTTTCGAGATAAGCCAACAAGAGCAACAGATTGCATTATCGGTGAGGTGGGCCTC
ACTGGAGAAATAAGAAGAGTTTCAAGAATTGAACAGCGAGTCCAGGAGGCTGCGAAACTAGGATTTGAGCGTATTATTTT
ACCAGCGAATAATTTGAGCGGCTGGACAGGTCCTAAAAATGTTCAATTGATTGGCGTTCACTCGGTTAGTGAAGCACTTG
AAGCTGCATTAGGAGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.79

100

0.771
  radA Streptococcus mitis NCTC 12261

63.319

99.782

0.632
  radA Streptococcus pneumoniae D39

63.736

99.129

0.632
  radA Streptococcus pneumoniae TIGR4

63.736

99.129

0.632
  radA Streptococcus pneumoniae R6

63.736

99.129

0.632
  radA Streptococcus pneumoniae Rx1

63.736

99.129

0.632
  radA Streptococcus mitis SK321

63.516

99.129

0.63

Multiple sequence alignment