Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MKY08_RS21675 Genome accession   NZ_CP151996
Coordinates   4434297..4435673 (-) Length   458 a.a.
NCBI ID   WP_069514289.1    Uniprot ID   -
Organism   Lysinibacillus sp. FSL M8-0337     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4429297..4440673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY08_RS21655 (MKY08_21655) gltX 4429717..4431177 (-) 1461 WP_069514298.1 glutamate--tRNA ligase -
  MKY08_RS21660 (MKY08_21660) ispF 4431302..4431778 (-) 477 WP_024364393.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MKY08_RS21665 (MKY08_21665) ispD 4432202..4432897 (-) 696 WP_069514295.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MKY08_RS21670 (MKY08_21670) - 4432910..4433995 (-) 1086 WP_069514292.1 PIN/TRAM domain-containing protein -
  MKY08_RS21675 (MKY08_21675) radA 4434297..4435673 (-) 1377 WP_069514289.1 DNA repair protein RadA Machinery gene
  MKY08_RS21680 (MKY08_21680) - 4435784..4438228 (-) 2445 WP_069514286.1 ATP-dependent Clp protease ATP-binding subunit -
  MKY08_RS21685 (MKY08_21685) - 4438218..4439327 (-) 1110 WP_069514283.1 protein arginine kinase -
  MKY08_RS21690 (MKY08_21690) - 4439324..4439866 (-) 543 WP_069514281.1 UvrB/UvrC motif-containing protein -
  MKY08_RS21695 (MKY08_21695) - 4439880..4440350 (-) 471 WP_024364400.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50001.81 Da        Isoelectric Point: 7.3824

>NTDB_id=984099 MKY08_RS21675 WP_069514289.1 4434297..4435673(-) (radA) [Lysinibacillus sp. FSL M8-0337]
MAKKKTKFVCTGCGYESAKWMGRCPGCGEWNKMVEEVEVVAKGPRGAFQHSATVTQKAVPIIQVEAAEESRVATEMGELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVVSQELYIYSETNLELLNQ
TIDDVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRGTDCFIGEVGLTG
EVRRVTRIEQRVLEAAKLGFKRAFIPASNIGGWDFPPGIEIVGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=984099 MKY08_RS21675 WP_069514289.1 4434297..4435673(-) (radA) [Lysinibacillus sp. FSL M8-0337]
ATGGCGAAGAAAAAAACTAAATTTGTCTGTACAGGTTGTGGCTATGAATCTGCAAAGTGGATGGGACGCTGTCCAGGATG
TGGTGAATGGAATAAAATGGTTGAGGAAGTAGAGGTTGTGGCGAAAGGTCCAAGAGGGGCATTCCAGCACTCTGCAACTG
TCACGCAAAAGGCTGTACCGATTATCCAAGTAGAAGCGGCTGAGGAGTCTCGGGTCGCTACAGAAATGGGTGAGCTCAAT
CGTGTCTTGGGTGGAGGAATTGTTCCTGGATCTCTGGTGTTAATCGGGGGTGATCCGGGTATCGGAAAATCGACATTGCT
ATTACAGGTGTCTGCGTTGTTGTCCAATAAAGGTCATCGCGTGCTCTATATTTCTGGTGAGGAATCCATTCGACAAACGA
AACTACGTGCAGAGCGTTTAGGGGTTGTATCTCAAGAGCTGTATATTTATTCGGAGACGAATTTAGAGTTGTTAAATCAA
ACAATCGACGATGTACAACCAAAGTTTGTGATTGTGGATTCTATCCAAACGGTGTTCCATCCAGAGGTGACAAGTGCGCC
AGGTAGTGTATCACAAGTACGTGAATGTACAGCGGAGCTAATGCGGATAGCGAAAACAAAAGGCATTGCCATTTTCTTAG
TTGGGCATGTGACGAAGGAAGGGCAAATTGCAGGACCACGTATTTTAGAGCATATGGTTGATACAGTGCTGTATTTCGAA
GGAGAACGACATCACAATCACCGTATTTTACGGAGCCAAAAAAACCGCTTTGGTTCTACAAATGAAATTGCTATCTTTGA
AATGCTTCAGGGGGGCTTAAAGGAAGTTTTGAATCCGTCTGAACTATTTTTACAGGAACGTTCACAAGGGGCAGCAGGTT
CAACAATCGTGGCATCAATGGAGGGAACAAGGCCAATTTTAGTAGAAATCCAATCACTTGTAACCCCTACAAGCTTTAAT
TATCCGAAACGGATGGCAACAGGAGTGGACCAAAATCGTGTGCAATTGCTTATGGCAGTGCTTGAAAAGCGCATGGGTCT
CATGTTACAAGCACAGGATGCATATATTAAAGTTGCTGGCGGAGTAAAGCTAGATGAGCCTGCTATTGACTTAGCTGTCT
TGACGAGTATTGTTTCTAGCTTTAAGGATCAGGCAGTACGGGGAACAGATTGCTTTATTGGAGAAGTAGGGTTAACAGGA
GAGGTTCGTCGTGTTACACGAATTGAACAACGTGTGTTAGAGGCGGCAAAACTAGGATTTAAGCGAGCATTTATCCCTGC
ATCTAATATTGGTGGTTGGGATTTCCCGCCTGGTATTGAAATTGTTGGTGTGGAAACAATTAAAGATGCATTAAATGCCT
GCTTTAGAGAGCTATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)

AAA domain (AAA_25)

(65-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.264

99.127

0.697
  radA Streptococcus pneumoniae Rx1

62.281

99.563

0.62
  radA Streptococcus pneumoniae D39

62.281

99.563

0.62
  radA Streptococcus pneumoniae R6

62.281

99.563

0.62
  radA Streptococcus pneumoniae TIGR4

62.281

99.563

0.62
  radA Streptococcus mitis NCTC 12261

62.281

99.563

0.62
  radA Streptococcus mitis SK321

62.281

99.563

0.62

Multiple sequence alignment