Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   NST73_RS09100 Genome accession   NZ_CP151989
Coordinates   1788714..1789094 (+) Length   126 a.a.
NCBI ID   WP_224559893.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1034     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1754724..1793506 1788714..1789094 within 0


Gene organization within MGE regions


Location: 1754724..1793506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST73_RS08930 (NST73_08930) - 1754724..1754984 (-) 261 WP_024720407.1 YesK family protein -
  NST73_RS08935 (NST73_08935) - 1755019..1755438 (-) 420 WP_024720408.1 hypothetical protein -
  NST73_RS08940 (NST73_08940) - 1755629..1756474 (+) 846 WP_284725267.1 SMI1/KNR4 family protein -
  NST73_RS08945 (NST73_08945) - 1756873..1757982 (+) 1110 WP_039166523.1 RapH N-terminal domain-containing protein -
  NST73_RS08950 (NST73_08950) - 1757983..1758117 (+) 135 WP_259254329.1 hypothetical protein -
  NST73_RS08955 (NST73_08955) - 1758206..1760131 (+) 1926 WP_144665462.1 T7SS effector LXG polymorphic toxin -
  NST73_RS08960 (NST73_08960) - 1760137..1760625 (+) 489 WP_144665464.1 immunity protein YezG family protein -
  NST73_RS08965 (NST73_08965) - 1760695..1761161 (-) 467 Protein_1718 macro domain-containing protein -
  NST73_RS08970 (NST73_08970) - 1761347..1761878 (+) 532 Protein_1719 GNAT family N-acetyltransferase -
  NST73_RS08975 (NST73_08975) - 1762019..1762600 (+) 582 WP_342499525.1 undecaprenyl-diphosphatase -
  NST73_RS08980 (NST73_08980) - 1762658..1763029 (-) 372 WP_024720418.1 iron chaperone -
  NST73_RS08985 (NST73_08985) - 1763415..1764068 (+) 654 WP_096881450.1 CotZ-related putative spore coat protein -
  NST73_RS08990 (NST73_08990) - 1764143..1764487 (+) 345 WP_008343952.1 S-Ena type endospore appendage -
  NST73_RS08995 (NST73_08995) - 1764532..1765605 (+) 1074 WP_342499526.1 collagen-like protein -
  NST73_RS09000 (NST73_09000) - 1765851..1766963 (+) 1113 WP_342499527.1 tetratricopeptide repeat protein -
  NST73_RS09005 (NST73_09005) - 1767427..1767648 (+) 222 WP_178930202.1 hypothetical protein -
  NST73_RS09010 (NST73_09010) - 1767687..1768382 (-) 696 WP_008343947.1 YoaK family protein -
  NST73_RS09015 (NST73_09015) - 1768417..1770282 (-) 1866 WP_342499528.1 DNA ligase D -
  NST73_RS09020 (NST73_09020) - 1770398..1771246 (+) 849 WP_035391954.1 Ku protein -
  NST73_RS09025 (NST73_09025) - 1771275..1771718 (-) 444 WP_007499382.1 DUF2188 domain-containing protein -
  NST73_RS09030 (NST73_09030) - 1771878..1772351 (+) 474 WP_053215847.1 VOC family protein -
  NST73_RS09035 (NST73_09035) - 1772400..1772813 (+) 414 WP_144678744.1 Rrf2 family transcriptional regulator -
  NST73_RS09040 (NST73_09040) - 1772949..1773797 (+) 849 WP_342499529.1 SDR family oxidoreductase -
  NST73_RS09045 (NST73_09045) - 1773990..1774706 (+) 717 WP_007499377.1 (Fe-S)-binding protein -
  NST73_RS09050 (NST73_09050) - 1774728..1776152 (+) 1425 WP_342462572.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
  NST73_RS09055 (NST73_09055) - 1776149..1776871 (+) 723 WP_268363534.1 lactate utilization protein C -
  NST73_RS09060 (NST73_09060) - 1777004..1777912 (+) 909 WP_039167701.1 DMT family transporter -
  NST73_RS09065 (NST73_09065) - 1777926..1778117 (-) 192 WP_326242680.1 hypothetical protein -
  NST73_RS09070 (NST73_09070) - 1778225..1780441 (+) 2217 WP_342499530.1 RNA ligase -
  NST73_RS09075 (NST73_09075) - 1780506..1781216 (+) 711 WP_047946233.1 DUF421 domain-containing protein -
  NST73_RS09080 (NST73_09080) - 1781445..1782026 (+) 582 WP_058213736.1 TetR/AcrR family transcriptional regulator -
  NST73_RS09085 (NST73_09085) - 1782048..1785191 (+) 3144 WP_342499531.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  NST73_RS09090 (NST73_09090) - 1785437..1786345 (+) 909 WP_160757649.1 trypsin-like serine protease -
  NST73_RS09095 (NST73_09095) - 1786492..1788486 (+) 1995 WP_326242675.1 glycosyltransferase family 39 protein -
  NST73_RS09100 (NST73_09100) nucA/comI 1788714..1789094 (+) 381 WP_224559893.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST73_RS09105 (NST73_09105) - 1789239..1790087 (+) 849 WP_017359585.1 STAS domain-containing protein -
  NST73_RS09110 (NST73_09110) - 1790120..1790662 (-) 543 WP_017359584.1 IseA DL-endopeptidase inhibitor family protein -
  NST73_RS09115 (NST73_09115) - 1790959..1791402 (+) 444 WP_144678736.1 hypothetical protein -
  NST73_RS09120 (NST73_09120) lexA 1791450..1792070 (-) 621 WP_326242672.1 transcriptional repressor LexA -
  NST73_RS09125 (NST73_09125) yneA 1792230..1792541 (+) 312 WP_007499348.1 cell division suppressor protein YneA -
  NST73_RS09130 (NST73_09130) - 1792559..1793206 (+) 648 WP_017359583.1 recombinase family protein -
  NST73_RS09135 (NST73_09135) - 1793276..1793506 (+) 231 WP_061419189.1 DUF896 domain-containing protein -

Sequence


Protein


Download         Length: 126 a.a.        Molecular weight: 13758.38 Da        Isoelectric Point: 7.9019

>NTDB_id=983739 NST73_RS09100 WP_224559893.1 1788714..1789094(+) (nucA/comI) [Bacillus sp. FSL W7-1034]
MGALLGGFGEDRSAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=983739 NST73_RS09100 WP_224559893.1 1788714..1789094(+) (nucA/comI) [Bacillus sp. FSL W7-1034]
ATGGGTGCGTTATTAGGTGGTTTTGGAGAGGATCGTTCCGCAAAAGGAGCAGATCGTTATGATCATGTGATTCAATTTCC
TAAGGAACGGTATCCTGAAACAGGCAGTCATATTCAAGAAGCTATTCGAAAAGGGCACTCAGACGTGTGTACCATTGACC
GAAATGGGGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCCACAAAACCTGGTTTTGACCGGGATGAATGGCCG
ATGGCGGTGTGTCTTGAAGGAGGAAAGGGGGCAAGCGTTCAATATGTCAGTCCATCAGATAATAGAGGCGCTGGTTCATG
GGTCGGGCATCAAATCAGCGGGTATCCTGACGGGAAACGAATTTTATTTATTGTCAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.824

94.444

0.556


Multiple sequence alignment