Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   NST09_RS11500 Genome accession   NZ_CP151988
Coordinates   2235404..2235697 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL W7-1346     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2230404..2240697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST09_RS11450 (NST09_11450) sinR 2230568..2230903 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  NST09_RS11455 (NST09_11455) - 2230971..2231771 (-) 801 WP_007501219.1 TasA family protein -
  NST09_RS11460 (NST09_11460) - 2231830..2232402 (-) 573 WP_017357979.1 signal peptidase I -
  NST09_RS11465 (NST09_11465) tapA 2232452..2232979 (-) 528 WP_041091748.1 amyloid fiber anchoring/assembly protein TapA -
  NST09_RS11470 (NST09_11470) - 2233232..2233558 (+) 327 WP_007501224.1 DUF3889 domain-containing protein -
  NST09_RS11475 (NST09_11475) - 2233593..2233787 (-) 195 WP_008342191.1 YqzE family protein -
  NST09_RS11480 (NST09_11480) comGG 2233848..2234231 (-) 384 WP_041091746.1 competence type IV pilus minor pilin ComGG -
  NST09_RS11485 (NST09_11485) comGF 2234228..2234725 (-) 498 WP_041091745.1 competence type IV pilus minor pilin ComGF -
  NST09_RS11490 (NST09_11490) comGE 2234667..2234981 (-) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  NST09_RS11495 (NST09_11495) comGD 2234965..2235411 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  NST09_RS11500 (NST09_11500) comGC 2235404..2235697 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NST09_RS11505 (NST09_11505) comGB 2235714..2236754 (-) 1041 WP_048001386.1 competence type IV pilus assembly protein ComGB -
  NST09_RS11510 (NST09_11510) comGA 2236735..2237805 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  NST09_RS11515 (NST09_11515) - 2238258..2239130 (-) 873 WP_035390819.1 STAS domain-containing protein -
  NST09_RS11525 (NST09_11525) - 2239356..2239736 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST09_RS11530 (NST09_11530) - 2239949..2240191 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=983682 NST09_RS11500 WP_008342186.1 2235404..2235697(-) (comGC) [Bacillus sp. FSL W7-1346]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=983682 NST09_RS11500 WP_008342186.1 2235404..2235697(-) (comGC) [Bacillus sp. FSL W7-1346]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAAGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment