Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NSQ11_RS05300 Genome accession   NZ_CP151987
Coordinates   1047999..1051703 (+) Length   1234 a.a.
NCBI ID   WP_326114221.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1582     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1042999..1056703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ11_RS05285 (NSQ11_05285) - 1043297..1043707 (+) 411 WP_008348626.1 DoxX family protein -
  NSQ11_RS05290 (NSQ11_05290) - 1043899..1044345 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  NSQ11_RS05295 (NSQ11_05295) addB 1044512..1048021 (+) 3510 WP_048001717.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSQ11_RS05300 (NSQ11_05300) addA 1047999..1051703 (+) 3705 WP_326114221.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSQ11_RS05305 (NSQ11_05305) sbcD 1051729..1052904 (+) 1176 WP_052320664.1 exonuclease subunit SbcD -
  NSQ11_RS05310 (NSQ11_05310) sbcC 1052901..1056296 (+) 3396 WP_342501289.1 exonuclease subunit SbcC -
  NSQ11_RS05315 (NSQ11_05315) - 1056314..1056613 (+) 300 WP_035700204.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142673.69 Da        Isoelectric Point: 5.2160

>NTDB_id=983599 NSQ11_RS05300 WP_326114221.1 1047999..1051703(+) (addA) [Bacillus sp. FSL W7-1582]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYKIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKEDSNETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTSTEMLLVHLDQHDTESDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LKEDMSLEKQKQDEVVQALMEGVPVQGYGDYEEEVKKRLTWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSVRKAR
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAILKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=983599 NSQ11_RS05300 WP_326114221.1 1047999..1051703(+) (addA) [Bacillus sp. FSL W7-1582]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTCATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGTTCCCTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTGCAAGTTCTCCGTACCTTTTATTATAAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCACATCCAGCGCCCGAACAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAGTTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGAACACTTTATGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCGGCTCTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGATCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAGAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCTGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAGTACTATCAACAGCAA
TTTGAAGAAGTACTCGTGGATGAATACCAGGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGAAA
ACATCCTGCCGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGAAGACAGCAATGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGT
AGCCGGGCTGATGTTTTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACGGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGTCGACAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAGCGATGAAGAACGAGAAGAGCTAGAGACGGTTCAATTTGAGGCGAGAGTGATTGCAAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGTTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGATCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGTTATTTTGAAGCTACAGAAGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTTGCTGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACCAGTGA
TAAAAAAGGCACTTATTATGACGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACGTGCAAAAAGCTGG
AGCGTTTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGTAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGACTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTATTTACTGCAGGACTTGGCAGGAACTTTAACATGATGGATTTAAACCAGTCCTATTTGCTCGA
TAAAGAATTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACCTTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAACTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAGGAAAAGTTA
TTTTTAGTCGGCTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGATGACTGGCTTCT
TCCTGATTTTGAACGATATCAAGCGAGAACATACTTAGATTTTATTGGCCCAGCCCTTATCCGTCATCAAGCGATGTCTT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCGCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTAAAAGAAGACATGTCACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGTGTGCCTGTTCAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAAAAAGACTTACATGGCGTTATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCTGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTTCTTCCGTTCGAAAAGCTCGT
GCGACATTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAGGCGATATTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.936

100

0.671

Multiple sequence alignment