Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NSS80_RS12695 Genome accession   NZ_CP151985
Coordinates   2427201..2427971 (-) Length   256 a.a.
NCBI ID   WP_101676866.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0350     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2422201..2432971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS80_RS12675 (NSS80_12675) - 2422293..2423114 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  NSS80_RS12680 (NSS80_12680) - 2423140..2423778 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  NSS80_RS12685 (NSS80_12685) glyS 2423946..2425991 (-) 2046 WP_024423018.1 glycine--tRNA ligase subunit beta -
  NSS80_RS12690 (NSS80_12690) glyQ 2425984..2426871 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  NSS80_RS12695 (NSS80_12695) recO 2427201..2427971 (-) 771 WP_101676866.1 DNA repair protein RecO Machinery gene
  NSS80_RS12700 (NSS80_12700) - 2428007..2428150 (-) 144 WP_003217288.1 YqzL family protein -
  NSS80_RS12705 (NSS80_12705) era 2428299..2429204 (-) 906 WP_007501305.1 GTPase Era -
  NSS80_RS12710 (NSS80_12710) - 2429197..2429595 (-) 399 WP_008356263.1 cytidine deaminase -
  NSS80_RS12715 (NSS80_12715) - 2429709..2430095 (-) 387 WP_024423020.1 diacylglycerol kinase family protein -
  NSS80_RS12720 (NSS80_12720) ybeY 2430073..2430552 (-) 480 WP_024423021.1 rRNA maturation RNase YbeY -
  NSS80_RS12725 (NSS80_12725) - 2430553..2432667 (-) 2115 WP_164512169.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29297.92 Da        Isoelectric Point: 8.1204

>NTDB_id=983500 NSS80_RS12695 WP_101676866.1 2427201..2427971(-) (recO) [Bacillus sp. FSL M8-0350]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELMDKGTEEKKSNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKQQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=983500 NSS80_RS12695 WP_101676866.1 2427201..2427971(-) (recO) [Bacillus sp. FSL M8-0350]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGTGTGATGGCCAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGCACCTTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGGCC
ATTCGGGAAGACTTATTTCTCACCGCATATGCCGCATATATGACGGAATTGATGGATAAAGGGACAGAAGAAAAAAAGTC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAACAGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75

100

0.75

Multiple sequence alignment