Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NSS80_RS12420 Genome accession   NZ_CP151985
Coordinates   2377626..2378696 (-) Length   356 a.a.
NCBI ID   WP_025092972.1    Uniprot ID   A0A5C0WKU5
Organism   Bacillus sp. FSL M8-0350     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2372626..2383696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS80_RS12370 (NSS80_12370) - 2372720..2373292 (-) 573 WP_024422971.1 signal peptidase I -
  NSS80_RS12375 (NSS80_12375) tapA 2373345..2373872 (-) 528 WP_234107214.1 amyloid fiber anchoring/assembly protein TapA -
  NSS80_RS12380 (NSS80_12380) - 2374172..2374450 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  NSS80_RS12385 (NSS80_12385) - 2374485..2374679 (-) 195 WP_056767455.1 YqzE family protein -
  NSS80_RS12390 (NSS80_12390) comGG 2374739..2375122 (-) 384 WP_268415633.1 competence type IV pilus minor pilin ComGG -
  NSS80_RS12395 (NSS80_12395) comGF 2375119..2375571 (-) 453 WP_025092975.1 competence type IV pilus minor pilin ComGF -
  NSS80_RS12400 (NSS80_12400) comGE 2375558..2375872 (-) 315 WP_087978326.1 competence type IV pilus minor pilin ComGE -
  NSS80_RS12405 (NSS80_12405) comGD 2375856..2376302 (-) 447 WP_268437366.1 competence type IV pilus minor pilin ComGD -
  NSS80_RS12410 (NSS80_12410) comGC 2376295..2376588 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  NSS80_RS12415 (NSS80_12415) comGB 2376605..2377645 (-) 1041 WP_024427986.1 competence type IV pilus assembly protein ComGB -
  NSS80_RS12420 (NSS80_12420) comGA 2377626..2378696 (-) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  NSS80_RS12425 (NSS80_12425) - 2378874..2379749 (-) 876 WP_317221936.1 STAS domain-containing protein -
  NSS80_RS12435 (NSS80_12435) - 2379974..2380354 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NSS80_RS12440 (NSS80_12440) - 2380568..2380810 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NSS80_RS12445 (NSS80_12445) - 2380858..2381496 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  NSS80_RS12450 (NSS80_12450) - 2381654..2381827 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NSS80_RS12455 (NSS80_12455) - 2382052..2382366 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  NSS80_RS12460 (NSS80_12460) - 2382443..2383696 (-) 1254 WP_041117981.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40412.86 Da        Isoelectric Point: 9.5360

>NTDB_id=983499 NSS80_RS12420 WP_025092972.1 2377626..2378696(-) (comGA) [Bacillus sp. FSL M8-0350]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=983499 NSS80_RS12420 WP_025092972.1 2377626..2378696(-) (comGA) [Bacillus sp. FSL M8-0350]
TTGTATGGGATTGAATATTTAAGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTGGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCAGGGAAAACAACAACACTATATTCACTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATATTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTATCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WKU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment