Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST65_RS08525 Genome accession   NZ_CP151983
Coordinates   1693949..1694329 (+) Length   126 a.a.
NCBI ID   WP_044335941.1    Uniprot ID   A0AAP7UKB4
Organism   Bacillus sp. FSL P4-0248     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1688949..1699329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST65_RS08515 (NST65_08515) - 1689444..1692587 (+) 3144 WP_211044056.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  NST65_RS08520 (NST65_08520) - 1692781..1693689 (+) 909 WP_024425124.1 trypsin-like serine protease -
  NST65_RS08525 (NST65_08525) nucA/comI 1693949..1694329 (+) 381 WP_044335941.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST65_RS08530 (NST65_08530) - 1694475..1695323 (+) 849 WP_041117519.1 STAS domain-containing protein -
  NST65_RS08535 (NST65_08535) - 1695356..1695898 (-) 543 WP_041109224.1 IseA DL-endopeptidase inhibitor family protein -
  NST65_RS08540 (NST65_08540) - 1696195..1696629 (+) 435 WP_111927431.1 hypothetical protein -
  NST65_RS08545 (NST65_08545) lexA 1696675..1697295 (-) 621 WP_034283422.1 transcriptional repressor LexA -
  NST65_RS08550 (NST65_08550) yneA 1697454..1697765 (+) 312 WP_111927432.1 cell division suppressor protein YneA -
  NST65_RS08555 (NST65_08555) - 1697783..1698430 (+) 648 WP_034283427.1 recombinase family protein -
  NST65_RS08560 (NST65_08560) - 1698500..1698730 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13727.41 Da        Isoelectric Point: 8.5068

>NTDB_id=983370 NST65_RS08525 WP_044335941.1 1693949..1694329(+) (nucA/comI) [Bacillus sp. FSL P4-0248]
MGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=983370 NST65_RS08525 WP_044335941.1 1693949..1694329(+) (nucA/comI) [Bacillus sp. FSL P4-0248]
ATGGGCACGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTACAATATGTTAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment