Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST65_RS01910 Genome accession   NZ_CP151983
Coordinates   358991..359419 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. FSL P4-0248     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 353991..364419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST65_RS01880 (NST65_01880) - 354153..354482 (+) 330 WP_024424311.1 YckD family protein -
  NST65_RS01885 (NST65_01885) - 354526..355290 (-) 765 WP_111927549.1 MBL fold metallo-hydrolase -
  NST65_RS01890 (NST65_01890) - 355406..355822 (+) 417 WP_181566871.1 Lrp/AsnC family transcriptional regulator -
  NST65_RS01895 (NST65_01895) - 355851..356657 (+) 807 WP_034283301.1 AAC(3) family N-acetyltransferase -
  NST65_RS01900 (NST65_01900) - 356659..357045 (-) 387 WP_046313809.1 competence protein ComJ -
  NST65_RS01905 (NST65_01905) - 357166..358863 (-) 1698 WP_046313811.1 methyl-accepting chemotaxis protein -
  NST65_RS01910 (NST65_01910) nucA/comI 358991..359419 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST65_RS01915 (NST65_01915) - 359787..361856 (+) 2070 WP_070326675.1 hydantoinase/oxoprolinase family protein -
  NST65_RS01920 (NST65_01920) - 361846..363834 (+) 1989 WP_111927547.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=983345 NST65_RS01910 WP_024424305.1 358991..359419(-) (nucA/comI) [Bacillus sp. FSL P4-0248]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=983345 NST65_RS01910 WP_024424305.1 358991..359419(-) (nucA/comI) [Bacillus sp. FSL P4-0248]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCGATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCAGACG
GCACAAGAATCCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment