Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NSQ25_RS11655 Genome accession   NZ_CP151982
Coordinates   2271501..2272271 (-) Length   256 a.a.
NCBI ID   WP_034280128.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0512     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2266501..2277271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ25_RS11635 (NSQ25_11635) - 2266594..2267415 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  NSQ25_RS11640 (NSQ25_11640) - 2267441..2268079 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  NSQ25_RS11645 (NSQ25_11645) glyS 2268247..2270292 (-) 2046 WP_120208221.1 glycine--tRNA ligase subunit beta -
  NSQ25_RS11650 (NSQ25_11650) glyQ 2270285..2271172 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  NSQ25_RS11655 (NSQ25_11655) recO 2271501..2272271 (-) 771 WP_034280128.1 DNA repair protein RecO Machinery gene
  NSQ25_RS11660 (NSQ25_11660) - 2272307..2272450 (-) 144 WP_003217288.1 YqzL family protein -
  NSQ25_RS11665 (NSQ25_11665) era 2272599..2273504 (-) 906 WP_326136232.1 GTPase Era -
  NSQ25_RS11670 (NSQ25_11670) - 2273497..2273895 (-) 399 WP_008356263.1 cytidine deaminase -
  NSQ25_RS11675 (NSQ25_11675) - 2274009..2274395 (-) 387 WP_024423020.1 diacylglycerol kinase family protein -
  NSQ25_RS11680 (NSQ25_11680) ybeY 2274373..2274852 (-) 480 WP_024423021.1 rRNA maturation RNase YbeY -
  NSQ25_RS11685 (NSQ25_11685) - 2274853..2276940 (-) 2088 WP_326136655.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29249.92 Da        Isoelectric Point: 8.4348

>NTDB_id=983316 NSQ25_RS11655 WP_034280128.1 2271501..2272271(-) (recO) [Bacillus sp. FSL R5-0512]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELMDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKQQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=983316 NSQ25_RS11655 WP_034280128.1 2271501..2272271(-) (recO) [Bacillus sp. FSL R5-0512]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGCGTGATGGCAAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCTATAAGCCAGCCGTTTT
TATATGGCACCTTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGGCC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTGATGGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGTACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTGCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCAATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAACAGCAGA
CGAAACAAGAAATTCGGCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment