Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NSQ25_RS08685 Genome accession   NZ_CP151982
Coordinates   1715364..1715744 (+) Length   126 a.a.
NCBI ID   WP_044335941.1    Uniprot ID   A0AAP7UKB4
Organism   Bacillus sp. FSL R5-0512     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1710364..1720744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ25_RS08675 (NSQ25_08675) - 1710860..1714003 (+) 3144 WP_326136383.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  NSQ25_RS08680 (NSQ25_08680) - 1714196..1715104 (+) 909 WP_326136381.1 trypsin-like serine protease -
  NSQ25_RS08685 (NSQ25_08685) nucA/comI 1715364..1715744 (+) 381 WP_044335941.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NSQ25_RS08690 (NSQ25_08690) - 1715890..1716738 (+) 849 WP_326136380.1 STAS domain-containing protein -
  NSQ25_RS08695 (NSQ25_08695) - 1716770..1717312 (-) 543 WP_326136378.1 IseA DL-endopeptidase inhibitor family protein -
  NSQ25_RS08700 (NSQ25_08700) - 1717608..1718060 (+) 453 WP_070325869.1 hypothetical protein -
  NSQ25_RS08705 (NSQ25_08705) lexA 1718106..1718726 (-) 621 WP_034283422.1 transcriptional repressor LexA -
  NSQ25_RS08710 (NSQ25_08710) yneA 1718885..1719196 (+) 312 WP_034283425.1 cell division suppressor protein YneA -
  NSQ25_RS08715 (NSQ25_08715) - 1719214..1719861 (+) 648 WP_034283427.1 recombinase family protein -
  NSQ25_RS08720 (NSQ25_08720) - 1719931..1720161 (+) 231 WP_034283429.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13727.41 Da        Isoelectric Point: 8.5068

>NTDB_id=983309 NSQ25_RS08685 WP_044335941.1 1715364..1715744(+) (nucA/comI) [Bacillus sp. FSL R5-0512]
MGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=983309 NSQ25_RS08685 WP_044335941.1 1715364..1715744(+) (nucA/comI) [Bacillus sp. FSL R5-0512]
ATGGGCACGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTACAATATGTTAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment